Fig. 1 Engineered expression of PSMA variants on CD19 CAR T cells.

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Presentation transcript:

Fig. 1 Engineered expression of PSMA variants on CD19 CAR T cells. Engineered expression of PSMA variants on CD19 CAR T cells. (A) CAR construct illustration demonstrating the CD19-targeting domain, costimulatory domain, and the location of the PSMA expression tag. Three different molecular constructs of PSMA were produced: WT, W2G, and N9del. VH, heavy chain variable domain; VL, light chain variable domain; GST, glutathione S-transferase; IgG4, immunoglobulin G4. (B) CD19-EGFRt or CD19-PSMA CAR T cells were generated, and flow cytometry was performed to identify CAR+ and PSMA+ CD4 or CD8 T cells. Gating was based on the mock transduction control. (C) Surface expression of PSMA was assessed by flow cytometry. Geometric mean fluorescent intensity (gMFI) is reported after gating on PSMA+ cells. (D) Mock, CD19-EGFRt, or CD19-PSMA CAR T cells were incubated with K562-CD19-NLR target cells at a ratio of 4:1. Target cells were analyzed every 2 hours using an IncuCyte live cell imaging system. TM, transmembrane; NLR, NucLight Red. Il Minn et al. Sci Adv 2019;5:eaaw5096 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).