The number of islet antigen–specific T cells is reduced in CT-treated RIP-LCMV-GP mice. The number of islet antigen–specific T cells is reduced in CT-treated.

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The number of islet antigen–specific T cells is reduced in CT-treated RIP-LCMV-GP mice. The number of islet antigen–specific T cells is reduced in CT-treated RIP-LCMV-GP mice. At days 20 and 31 after infection, the spleen and pancreas of RIP-LCMV mice treated with isotype-matched control antibody, aCD3 and isotype-matched control antibody (aCD3), aCXCL10 alone, or CT were removed and the frequency of CD4 T cells, CD8 T cells, FoxP3+ CD4 T cells, and RIP-LCMV-GP33–specific CD8 T cells were analyzed by flow cytometry. The total numbers of infiltrating cells in the pancreas, rather than their relative frequencies, were determined. To this end, lymphocytes were isolated from the pancreas after removal of the pancreatic lymph nodes. A: Representative dot blots from flow cytometry of splenocytes and pancreatic lymphocytes harvested at day 31 after infection. Frequencies (spleen) and total numbers (pancreas) were calculated for total CD4 T cells, total CD8 T cells, FoxP3+ CD4 T cells, and RIP-LCMV-GP33–specific CD8 T cells at days 20 (B) and 31 (C) after infection. Data are mean values ± SD (day 20: n = 10; day 31: n = 6–13). Significant differences are indicated (P values). D: Calculated ratio of FoxP3+ CD4 T cells to RIP-LCMV–GP33–specific CD8 T cells in the spleen and pancreas at day 31 after infection. E and F: Islet antigen (RIP-LCMV-GP33)–specific CD8 T cells were further analyzed for TNF-α expression in splenocytes and pancreatic lymphocytes at day 20 after infection. Representative dot blots (E) and the calculated ratio of TNF-α–producing islet antigen–specific CD8 T cells (F) are displayed. Data are mean values ± SD (n = 5). Significant differences are indicated (P values). C, control; Teff, effector T cell. Stanley Lasch et al. Diabetes 2015;64:4198-4211 ©2015 by American Diabetes Association