Microarray in the offspring of T2D parents and age-matched control subjects. Microarray in the offspring of T2D parents and age-matched control subjects.

Slides:

Advertisements

Similar presentations

ΑKAP is an anchoring protein for a novel CaM kinase II isoform in skeletal muscle by K. ‐ Ulrich Bayer, Klaus Harbers, and Howard Schulman EMBO J. Volume.

Advertisements

Network protein vulnerability in EGFR TKI‐resistant cells and specific for EGFR‐mutated cells. Network protein vulnerability in EGFR TKI‐resistant cells.

Heat Shock Protein Expression in Rat Skeletal Muscle After Repeated Applications of Pulsed and Continuous Ultrasound Ethne L. Nussbaum, PhD, PT, Marius.

HERV-K10s and Immune-Mediated (Type 1) Diabetes

Inhibition of ILK restores epithelial ZO-1 and E-cadherin and inhibits fibronectin and Snail1 expression after TGF-β1 treatment. Inhibition of ILK restores.

HIS-24 regulates expression of infection-inducible genes.

Figure 3. Genes differentially expressed in batch cultures during adaptation to low temperature. Genes differentially expressed in batch cultures during.

Microarray analysis of phospho-Twist1–responsive genes.

Success rates in validation of antibodies from external providers

Chromosome 8 cDNA microarray gene expression profile of the amplified regions of 8p11–12 in SUM-44, -52, and -225 cells versus MCF10A HME control cells.

Absence of ARMS-associated chromosomal translocation.

A genome-wide RNAi pooled screen identifies NF1 as a key determinant of RAF inhibitor sensitivity in melanoma cells. A genome-wide RNAi pooled screen identifies.

Effect of Nf1 knockdown on Wnt pathway in MSC80 cells.

HOXB7 interacts with ERα and enhances expression of ER target genes.

Box plots showing the relative difference in distribution of mtDNA copy number per cell between patients with ESRD and healthy control subjects frequency.

ZFAND5 expression is induced by dexamethasone, but not by heat shock or tunicamycin treatment. ZFAND5 expression is induced by dexamethasone, but not by.

HJURP is required for CENP-A localization to centromeres.

Reversal of MOR lysosomal targeting by silencing enhanced Rab7 expression. Reversal of MOR lysosomal targeting by silencing enhanced Rab7 expression. A:

Quantitation of Hsp-72 mRNA in human muscle biopsies and heat-shocked NIH-3T3 cells, using competitive RT-PCR. Quantitation of Hsp-72 mRNA in human muscle.

Validation of DNMT3A splice variant expression.

The risk allele of rs affects TCR-mediated intracellular calcium flux in CD4+ T cells from subjects with T1D or RA. Previously frozen PBMCs from.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

Time-dependent effects of IL-6 on tyrosine phosphorylation of IRS-1.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

IGF-II and IGFBP-5 rescue the atrophy of myotubes induced by adipocytes. IGF-II and IGFBP-5 rescue the atrophy of myotubes induced by adipocytes. A–D:

IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.

Late gestational SF exposures alter miR-103/107 expression profiles and their target protein Cav1 in VWAT of offspring mice. Late gestational SF exposures.

The p53 pathway is involved in the inhibition of cell proliferation observed in 15-LOX-1-overexpressing cells. The p53 pathway is involved in the inhibition.

Phosphorylation of AMPK-Thr172 before and after metformin treatment in muscle from subjects with type 2 diabetes. Phosphorylation of AMPK-Thr172 before.

Effects of NAT10 inhibition or depletion on gene expression.

EPA treatment rescued LPL RNAi–induced suppression of MTCOI protein expression. EPA treatment rescued LPL RNAi–induced suppression of MTCOI protein expression.

CML-HSA treatment activates MAPK family members ERK1/2 and p38 but not JNK. Samples were taken at the indicated times after 100 μg/ml CML-HSA exposure.

LPL knockdown by RNAi in RC13 human rhabdomyosarcoma cell line.

Expression of PKCδ in islets and other tissues of control and PKCδKN-overexpressing mice. Expression of PKCδ in islets and other tissues of control and.

Lipoprotein lipase is decreased in skeletal muscle of young, lean IR offspring of T2D parents. Lipoprotein lipase is decreased in skeletal muscle of young,

Chronic rapamycin treatment impairs β-cell mass and insulin clearance in rats. Chronic rapamycin treatment impairs β-cell mass and insulin clearance in.

IFN-γ prevents SAG-induced inhibition of adipocyte differentiation.

IKKβ protects adipocytes from HFD-induced cell death.

Difference in average school grades between offspring born to women with type 1 diabetes and the matched control children, by maternal third trimester.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

Effects of knockdown of DDR1 levels on proliferation and apoptosis.

Evaluation of top candidate clones for selective killing in bispecific antibody format. Evaluation of top candidate clones for selective killing in bispecific.

Imatinib mesylate inhibits PDGF-mediated ERK and Akt activation.

Heat map of genes for which CR significantly altered expression versus AL. Cluster analysis of genes significantly changed by the CR intervention compared.

Depletion of HDAC2 sensitizes cells to epirubicin-induced apoptosis.

A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

Src expression in a panel of human TCC cell lines.

Microarray analysis of global gene expression profiles of PC-3 and derivative cell lines. Microarray analysis of global gene expression profiles of PC-3.

Western blot analysis of plasma proteins from two-dimensional gel electrophoresis. Western blot analysis of plasma proteins from two-dimensional gel electrophoresis.

Analysis of mRNA and protein levels of Loricrin, COMP, CXCL9, KRT19, and CYP 3A5 genes in OSFs compared with normal controls. Analysis of mRNA and protein.

SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.

Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

The effects of HDAC2 knockdown on cell-cycle proteins.

Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Western blot analysis for bFGF expression in human glioma cell lines.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

MTB1 to MTB3 Are Direct Transcriptional Targets of MYC2.

miR-146a is highly expressed selectively on γδ27− T cells.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

T-cell expression of second-generation CARs

Association of TCTP with Pim-3 in human pancreatic cancer cell lines.

IL-1β expression is increased in MM cell lines by the co-culture with platelets in vitro. IL-1β expression is increased in MM cell lines by the co-culture.

WM volume did not show the expected increase in volume with age in children with type 1 diabetes (●), in contrast with HC subjects (▲) who showed the (expected)

H3K36me3 is not essential for LEDGF and MLL target gene occupancy.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Fig. 3. Association between peak CTL019 expansion and response.

TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3 pathway. TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3.

Presentation transcript:

Microarray in the offspring of T2D parents and age-matched control subjects. Microarray in the offspring of T2D parents and age-matched control subjects. A: Heat-map representations of genes detected in expression analysis of skeletal muscle of the offspring of T2D parents and age-matched control subjects. The list shows the top 100 genes by P value between two groups. B: Screening experiments using Mitotracker-to-DAPI ratio were performed after treatment with RNAi against 250 candidate genes in RC13 human rhabdomyosarcoma cell line. After the screening experiments, 45 genes were tested by Western blotting with MTCOI, PDH, and SDHA antibodies, and 15 genes showed significant change compared with treatment of nontargeting RNAi. C: Heat-map representations of overlapped genes in first microarray and second microarray. D: Six genes were overlapped with first microarray, second microarray, and former screening (B). A total of 2 out of 6 genes showed significant difference determined by RT-qPCR using TaqMan probe in control subjects (n = 7) and IR offspring (n = 13). Katsutaro Morino et al. Diabetes 2012;61:877-887 ©2012 by American Diabetes Association