Figure 3. Sensitivity and specificity of microarray analysis in relation to target number Sensitivity and specificity of microarray analysis in relation.

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Figure 3. Sensitivity and specificity of microarray analysis in relation to target number Sensitivity and specificity of microarray analysis in relation to target number (A) Comparison of overall assay accuracy (both sensitivity and specificity) when screening against an increasing number of target antigens. Assay specificity declines as the numbers of target antigens increases. Improved assay performance is achieved when a small panel of 10 targets is selected. (B) Dot plot compares the maximum binding intensity signal for each patient or control when comparing single and heteromeric complexes. GBS patient median arbitrary binding intensity for single targets (747.3 FIU) is significantly less than for heteromeric complex targets (3589.9 FIU; p < 0.0001), indicating that heteromeric complexes return higher signals than single antigens. Susan K. Halstead et al. Neurol Neuroimmunol Neuroinflamm 2016;3:e284 © 2016 American Academy of Neurology