Fig. 5. Serum estrogen concentration in rats following different treatments. 24 female Sprague–Dawley rats with ovariectomy underwent intraperitoneal administration.

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Fig. 5. Serum estrogen concentration in rats following different treatments. 24 female Sprague–Dawley rats with ovariectomy underwent intraperitoneal administration of estrogen or its vehicle. 12 rats of sham-operated group only underwent the resection of a bit of fat around bilateral ovaries. Serum estrogen concentration in rats following different treatments. 24 female Sprague–Dawley rats with ovariectomy underwent intraperitoneal administration of estrogen or its vehicle. 12 rats of sham-operated group only underwent the resection of a bit of fat around bilateral ovaries. All these rats were injected with 10 mg/kg NTG once a week and the injection was repeated five times. Rats' blood was drawn from the vena cava before the sacrifice, and then centrifuged at 3000 g for 10 min at 4°C to separate serum. Serum estrogen concentration was quantified by ELISA. **P<0.01 between each group (n=12, one-way ANOVA). Jiu-Qing Guo et al. Biology Open 2017;6:8-16 © 2017. Published by The Company of Biologists Ltd