An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome Martina.

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Presentation transcript:

An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome Martina Wallace, Ph.D., Evelyn Cottell, Ph.D., Michael J. Gibney, Ph.D., Fionnuala M. McAuliffe, M.D., Mary Wingfield, M.D., Lorraine Brennan, Ph.D. Fertility and Sterility Volume 97, Issue 5, Pages 1078-1084.e8 (May 2012) DOI: 10.1016/j.fertnstert.2012.01.122 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Partial least squares discriminant analysis (PLS-DA) scores plot of follicular fluid samples from follicles where the oocyte resulted in a one-cell embryo (n = 9, black box) or a two-cell embryo (n = 18, open diamond) on day 2 after fertilization. R2 = 0.502, Q2 = 0.39. The x (t[1]) and y (t[2]) represent the PLS components 1 and 2, respectively. The open diamond symbols correspond to samples from follicles where the oocyte resulted in a two-cell embryo. The black box symbols correspond to samples from follicles where the oocyte resulted in a one-cell embryo. Fertility and Sterility 2012 97, 1078-1084.e8DOI: (10.1016/j.fertnstert.2012.01.122) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Flowchart of the outcome of oocytes from follicles where follicular fluid was collected and used to investigate oocyte developmental potential. ∗Three samples within this group were of poor analytical quality. ∗∗One sample from this group was removed from further analysis due to poor analytical quality. Fertility and Sterility 2012 97, 1078-1084.e8DOI: (10.1016/j.fertnstert.2012.01.122) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Partial least squares discriminant analysis (PLS-DA) plot of follicular fluid samples from follicles where the oocyte resulted in a one-cell embryo (n = 9, boxes) or a three-cell embryo (n = 12, diamonds) on day 2 after fertilization. R2 = 0.517, Q2 = 0.318. Fertility and Sterility 2012 97, 1078-1084.e8DOI: (10.1016/j.fertnstert.2012.01.122) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 Partial least squares discriminant analysis (PLS-DA) plot of follicular fluid samples from follicles where the oocyte resulted in a one-cell embryo (n = 9, boxes) or a four-cell embryo (n = 13, triangles) on day 2 after fertilization. R2 = 0.471, Q2 = 0.306. Fertility and Sterility 2012 97, 1078-1084.e8DOI: (10.1016/j.fertnstert.2012.01.122) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 4 Receiver operating characteristic (ROC) analysis of the panel of biomarkers (lactate, glucose, proline, leucine, and isoleucine) identified as being predictive of pregnancy outcome. The ROC analysis was performed on the data used to build the original PLS-DA model (n = 22). Solid line: A sevenfold cross validation was performed to test the predictability (area under the curve = 0.775). Fertility and Sterility 2012 97, 1078-1084.e8DOI: (10.1016/j.fertnstert.2012.01.122) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 5 Flowchart of the outcome of oocytes from follicles where follicular fluid was collected and used to investigate embryo implantation potential. ∗Three samples and ∗∗one sample from these groups were removed from further analysis due to poor analytical quality. Fertility and Sterility 2012 97, 1078-1084.e8DOI: (10.1016/j.fertnstert.2012.01.122) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions