Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P

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Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P. pastoris A2AR–SMALP (●) and DDM-solubilized A2AR (▲) prepared from P. pastoris overexpressing A2aR were incubated for 30 min at the stated temperatures, chilled on ice, before specific binding o... Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P. pastoris A2AR–SMALP (●) and DDM-solubilized A2AR (▲) prepared from P. pastoris overexpressing A2aR were incubated for 30 min at the stated temperatures, chilled on ice, before specific binding of [3H]ZM241385 was determined as described in ‘Methods’. Data are expressed as specific binding relative to the 20°C data point (mean ± S.E.M. of three separate experiments performed in triplicate). Mohammed Jamshad et al. Biosci. Rep. 2015;35:e00188 ©2015 by Portland Press Ltd