Characteristics of a novel intronic enhancer human islet.

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Characteristics of a novel intronic enhancer human islet. Characteristics of a novel intronic enhancer human islet. A: The chromatin landscape for a putative enhancer in an intron of MEG3 is shown with human islet ChIP sequencing tracks for histone modification marks associated with enhancers (H3K4me1 and H3K27ac) and active promoters (H3K4me3). Occupancy of islet transcription factors PDX1, NKX2.2, and FOXA2 at the putative enhancer is also shown. Data retrieved from Pasquali et al. (15). B: Activity of the MEG3 enhancer was validated by luciferase reporter assays. pGL3 vectors with the MEG3-DMR and the enhancer sequence in either its native (For) or reverse (Rev) orientation were transfected into βTC6 cells. Data are represented as mean ± SEM. n = 3. P values calculated by Student t test. **P < 0.1 × 10−3; ***P < 0.1 × 10−5. Vasumathi Kameswaran et al. Diabetes 2018;67:1807-1815 ©2018 by American Diabetes Association