Effect of PDGF-BB on mesenchymal mesonephric cell migration.

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Supplementary Figure S1, Schneider et al lymphocytes monocytes medium WT sup KO sup FM ratio (%) medium WT sup KO sup medium WT sup KO sup.

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Effect of PDGF-BB on mesenchymal mesonephric cell migration. Effect of PDGF-BB on mesenchymal mesonephric cell migration. Assays were performed by incubating cells for 16-18 hours in cell culture inserts with 8 μm porosity membrane. Cells that had migrated to the lower surface of the filters were counted. (A) Dose-response curve. Various concentrations of PDGF-BB were added to the lower wells only. (B) Chemotaxis vs chemokinesis analysis. PDGF-BB (10 ng/ml) was added to the upper well, to the lower well, or to both set of wells. Results are mean±s.d. of three independent experiments, each performed in duplicate. *P<0.01 vs untreated control. Antonella Puglianiello et al. J Cell Sci 2004;117:1151-1160 © The Company of Biologists Limited 2004