Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted Drosophila S2 cells. Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted.

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Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted Drosophila S2 cells. Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted Drosophila S2 cells. In control cells during prometaphase and anaphase DmSMC4 and Barren co-localise to an axial structure of sister chromatids. After depletion of DmSMC4 by dsRNAi, chromosomal-associated DmSMC4 is significantly reduced after 48 hours and undetectable after 96 h. A reduction in the level of chromatin-associated DmSMC4 causes the formation of short chromosomes with unseparated sister chromatids and a highly abnormal distribution of Barren. In cells that show no DmSMC4 staining, chromosomes are short, no defined sister chromatids are observed and Barren is undetected. Scale bars: 5 μm. Paula A. Coelho et al. J Cell Sci 2003;116:4763-4776 © The Company of Biologists Limited 2003