Cell cycle release after M phase arrest (h)

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Cell cycle release after M phase arrest (h) Supplementary Fig. S6 a b ZNF313 p21WAF1 Cyclin D1 Cyclin B1 CDK6 Tubulin Serum addition (1 h) Serum withdrawal Thymidine block Nocodazole Control G1 S G2/M arrest Control Thymidine block Serum addition (1 h) Nocodazole Serum withdrawal c d Control 0 3 6 9 12 15 18 21 24 HeLa : M pahse arrest by thymidine-nocodazole-mitotic shake-off method. Cell cycle release after M phase arrest (h) C 0 3 6 9 12 15 18 21 24 (h) ZNF313 p21WAF1 Cyclin B1 Tubulin Hours after 10% FBS addition (HCT116) G1 S G2/M e f ZNF313 p21WAF1 Tubulin Control 10% FBS IGF-1 EGF FGF A549 pcDNA3.1 WT-ZNF313 HeLa HCT116 70.1 13.2 16.7 48.2 31.3 20.5 50.3 21.6 18.1 41.1 33.3 25.6 G1 (%) S G2/M 52.8 15.8 31.4 67.5 10.3 22.2 PC3 HCT116 siControl siZNF313-1 73.9 11.3 14.8 55.4 15.2 29.4