Intraperitoneal injection of tBHQ reduced sensorimotor deficit and stroke damage 24 h after ischemia-reperfusion. Intraperitoneal injection of tBHQ reduced sensorimotor deficit and stroke damage 24 h after ischemia-reperfusion. Sprague Dawley rats were injected with tBHQ intraperitoneally (16.7 mg/kg; 3 times) or vehicle, as described in Figure 3, followed by focal ischemia-reperfusion. The animals were killed 24 h after initiation of transient MCAO (1.5 h), and brain slices were stained with TTC. A, tBHQ treatment significantly reduced stroke damage in anterior cortical regions of the affected hemisphere. B, Similar to intracerebroventricular delivery of tBHQ, no sparing of subcortical tissue was observed. C, Acute sensorimotor deficits assessed during the stroke (1.5 h) were similar, suggesting that animals from both groups received the same degree of ischemia. However, sensorimotor deficit was significantly lower in the tBHQ-treated group when evaluated 24 h after stroke. Avg. Neurol. Score, Average neurological score. D, Average animal weight in the two treatment groups was not different when examined both 24 h after intraperitoneal injection of tBHQ and 24 h after stroke. E, A schematic diagram summarizing the injection and stroke timeline. The short arrows represent time points of tBHQ injection. Data represent mean ± SEM collected from n = 11 animals per treatment group. *p < 0.05 and **p < 0.01, two-tailed t test or one-way ANOVA with Bonferroni's post hoc test. Andy Y. Shih et al. J. Neurosci. 2005;25:10321-10335 ©2005 by Society for Neuroscience