Amplitudes of Ca2+ puffs evoked by different IP3R subtypes.

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Amplitudes of Ca2+ puffs evoked by different IP3R subtypes. Amplitudes of Ca2+ puffs evoked by different IP3R subtypes. (A) Examples of Ca2+ puffs from WT cells show discrete fluorescence steps in falling phase. Dashed lines show unitary steps. (B) Summary of results (mean±s.e.m. n=10 puffs from different cells) show the amplitude of unitary fluorescence steps during the falling phase of Ca2+ puffs. Values do not differ significantly between cell lines (P>0.05). (C) Frequency distributions (mean±s.e.m., n=15 cells) show the estimated number of active IP3Rs within each Ca2+ puff. The dashed line indicates the mean value for WT cells. (D) Summary of results (mean±s.e.m., n=15 cells) show the ΔF values from which the number of active IP3Rs within each Ca2+ puff was calculated. *P<0.05, relative to WT. Stefania Mataragka, and Colin W. Taylor J Cell Sci 2018;131:jcs220848 © 2018. Published by The Company of Biologists Ltd