Rhinovirus-induced PBMC responses and outcome of experimental infection in allergic subjects David E. Parry, MD*, William W. Busse, MD, Kris A. Sukow,

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Presentation transcript:

Rhinovirus-induced PBMC responses and outcome of experimental infection in allergic subjects David E. Parry, MD*, William W. Busse, MD, Kris A. Sukow, BS, Claire R. Dick, BS, Cheri Swenson, BS, James E. Gern, MD Journal of Allergy and Clinical Immunology Volume 105, Issue 4, Pages 692-698 (April 2000) DOI: 10.1067/mai.2000.104785 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 Association between RV shedding in nasal secretions and symptom scores. Cumulative daily quantitative viral titers (log TCID50 units/mL) of nasal secretions from first 3 days after inoculation with RV16 were compared with sum of daily symptom scores over same time period (n = 22). Journal of Allergy and Clinical Immunology 2000 105, 692-698DOI: (10.1067/mai.2000.104785) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 Relationship between peak RV shedding and changes in PBMC proliferative responses. Proliferation of PBMCs to tetanus or RV16 was determined just before and 7 and 28 days after inoculation with RV16. Cold-related changes in proliferative responses to RV16 (A, B) and tetanus (C, D) were calculated (Δlog [SI units]) for each time period and compared with peak viral shedding for each subject. Journal of Allergy and Clinical Immunology 2000 105, 692-698DOI: (10.1067/mai.2000.104785) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 Effect of precold PBMC proliferation on peak viral shedding after inoculation with RV16. Proliferative responses to RV16 were determined in PBMCs isolated from peripheral blood just before inoculation, and these responses were compared with the peak viral shedding measured in nasal lavage fluid after inoculation with RV16. Filled circles, Subjects who had no serologic evidence of prior RV16 infection at screening but had detectable neutralizing activity at inoculation (n = 7); open circles, subjects with no detectable neutralizing antibody to RV16 at either point in time (n = 14). Journal of Allergy and Clinical Immunology 2000 105, 692-698DOI: (10.1067/mai.2000.104785) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 4 Effect of RV16 on PBMC secretion of IFN-γ and IL-5. PBMCs were obtained from study subjects just before (precold, n = 17) and 28 days after (day 28, n = 22) inoculation with RV16. PBMCs were incubated ex vivo with RV16 or medium alone for 6 days, and culture supernates were analyzed for IFN-γ (A) and IL-5 (B).Filled circles, Geometric mean values. Asterisk, P < .001 versus control samples. Journal of Allergy and Clinical Immunology 2000 105, 692-698DOI: (10.1067/mai.2000.104785) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 5 Relationship of precold RV-induced IFN-γ to peak viral shedding after inoculation. PBMCs were obtained before inoculation with RV16, and samples were incubated for 6 days with either RV16 (107 PFU/mL) or medium alone as a control. Increases in RV-induced IFN-γ (RV-stimulated samples: control samples, Δlog units) were significantly associated with lower peak viral shedding after inoculation with RV16. Journal of Allergy and Clinical Immunology 2000 105, 692-698DOI: (10.1067/mai.2000.104785) Copyright © 2000 Mosby, Inc. Terms and Conditions