EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed.

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Presentation transcript:

EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. (A) Establishment of RasV12-transformed cells stably expressing EPLIN shRNA1. Knockdown of EPLIN protein was analyzed by western blotting (WB) using the anti-EPLIN antibody. Equal protein loading was confirmed using the anti-GAPDH antibody. (B) Knockdown of EPLIN suppresses the apical extrusion of RasV12-transformed cells. Normal MDCK cells were mixed with MDCK-pTR GFP–RasV12 cells (upper panels, green) or MDCK-pTR GFP–RasV12-EPLIN shRNA1 cells (lower panels, green), and incubated with tetracycline for 24 hours. Cells were stained with Hoechst 33342 (blue), anti-EPLIN antibody (red) and Alexa-Fluor-647-conjugated phalloidin (white). Scale bars: 10 µm. (C) Quantification of the apical extrusion for B. Data are the mean±s.d. from three independent experiments. *P<0.05; n = 89 and 102 cells. Atsuko Ohoka et al. J Cell Sci 2015;128:781-789 © 2015. Published by The Company of Biologists Ltd