Recording CAPs from mouse optic nerve.

Slides:



Advertisements
Similar presentations
Training begins in… 15:00 minutes Training begins in… 14:00 minutes.
Advertisements

Date of download: 7/8/2016 Copyright © 2016 SPIE. All rights reserved. ChR2-EYFP expression in transgenic mouse brain slices. (a) Fluorescent microscope.
Lixia Gao, Kevin Kostlan, Yunyan Wang, Xiaoqin Wang  Neuron 
Computation and classification by cultured neuronal networks.
Modeling of an isolated rhythm-generating population.
Effects of Arousal on Mouse Sensory Cortex Depend on Modality
A Cellular Mechanism for Prepulse Inhibition
A, ACPs establish direct synaptic contact with AOB GCs
PmCo feedback enhances MC responses to vomeronasal inputs.
Knut Debus, Manfred Lindau  Biophysical Journal 
Whole-cell patch-clamp analysis of NAc cells.
Vitamin D3 levels alter amphetamine-related behaviors in mice.
Synaptic transmission and plasticity is disturbed in 2-week-old but not in adult SynDIG1 β-gal mutant mice. Synaptic transmission and plasticity is disturbed.
Implicit adaptation generalizes around the aiming location (30° CCW)
A, Multivariate glm analysis for the aggregate observer (for the interval range within –450 and 250 ms from action execution). A, Multivariate glm analysis.
Computer simulation on a single myelinated fiber with the dimensions of the mouse optic nerve. Computer simulation on a single myelinated fiber with the.
Sparse excision of PirB at E15
Neuronal ATM level is reduced in mouse models of AD
Methods. Methods. A, Setup of the IR behavior chamber. Four reward ports line the walls of the circular chamber. The angle θ indicates how far apart adjacent.
Fig. 2. Kinetics of OptoEphB2 activation.
CNS administration of XPro1595 does not affect TMEV-induced acute seizure frequency and intensity. CNS administration of XPro1595 does not affect TMEV-induced.
CT and CI odor responses.
Modulation of high γ activity in different brain regions is correlated with behavior. Modulation of high γ activity in different brain regions is correlated.
Lixia Gao, Kevin Kostlan, Yunyan Wang, Xiaoqin Wang  Neuron 
IANX transiently increased cortical responses to maxillary molar pulp stimulation. IANX transiently increased cortical responses to maxillary molar pulp.
The adult female mouse ARC and VMH generate newborn neurons that respond to estrogens and leptin. The adult female mouse ARC and VMH generate newborn neurons.
Results of CFC training and testing.
Model of ILD sensitivity in the LSO. A, Circuit diagram.
Learning rates and CR latency data for mice trained at trace 50–350 or trace 50–450. Learning rates and CR latency data for mice trained at trace 50–350.
Template application to detect KC-like activity.
HFOs resulting from noisy input to pyramidal cell population.
c-Fos in RFMes GABAergic neurons across groups.
P450 inhibition does not alter the isotopologue-specific differential responses. P450 inhibition does not alter the isotopologue-specific differential.
No difference in the properties of mEPSCs of DGCs recorded in brain slices obtained from PBS-injected (control) and TMEV-infected mice during the acute.
Short-term synaptic depression in SCN neurons during stimulus train application. Short-term synaptic depression in SCN neurons during stimulus train application.
Analysis of individual full cells.
Purkinje cells inhibit large fastigial glycinergic neurons
SVs with delayed availability for release are derived from bulk and endosomes. SVs with delayed availability for release are derived from bulk and endosomes.
Spectra superimposed on the brain over each electrode for patient JC during a hand movement task (red curves) and resting interval (blue curves). Spectra.
PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus
Diagram depicting the location of electrode placement.
IANX transiently decreased the cortical responses to the mandibular molar pulp stimulation. IANX transiently decreased the cortical responses to the mandibular.
Closed-loop experiments to probe the range of stimulus sensitivity.
Light illumination activates primary afferents with conduction velocity in a range of Aβ fibers. Light illumination activates primary afferents with conduction.
Calibration of the voltage dependence of spine ΔF/F using optical recordings in spines of bAPs. Calibration of the voltage dependence of spine ΔF/F using.
Localization behavior in ITD-alone condition and head turn analysis.
Examples of single-trial, time-domain, bandpass-filtered (1–50 Hz) EEG traces at electrode Fz from phantom (cantaloupe) and human participants (one with.
Stimulation of VTA GABA neurons reduces firing activity in the DG in vivo. Stimulation of VTA GABA neurons reduces firing activity in the DG in vivo. A,
GABAARs in RFMes GABAergic neurons across groups.
AP-induced axonal swelling and subsequent migration of microglial processes to the periaxonal area. AP-induced axonal swelling and subsequent migration.
Expression of the snail c-Fos homolog.
Exposure of in vitro sensorimotor synaptic connections to RNA from trained animals enhanced the strength of a subpopulation of synapses. Exposure of in.
Elimination of dendritic spines per 2 d.
Power spectra of EMG during the pre- and post-feedback sessions.
E-stimulation of the olfactory nerve evokes PGC inhibitory responses in distant glomeruli. E-stimulation of the olfactory nerve evokes PGC inhibitory responses.
A, Grand averages of response-locked ERPs at midline electrodes in participants homozygous for the C and T allele (DRD4–521). A, Grand averages of response-locked.
Reduced twitch and tetanic force by nerve stimulation in HSA-Yap−/− mice. Reduced twitch and tetanic force by nerve stimulation in HSA-Yap−/− mice. A,
SSC LTP in narrow-field vertical neurons is induced by SO stimulation at 20 Hz for 20 s. sSC LTP in narrow-field vertical neurons is induced by SO stimulation.
Thy1S promoter drives sparse expression of hrGFP in marmoset cortex.
Effects of CNO on spontaneous locomotion and amphetamine-induced hyperlocomotion. Effects of CNO on spontaneous locomotion and amphetamine-induced hyperlocomotion.
Experimental design. Experimental design. Animals were randomized to stimulation groups according to amphetamine rotation results (Amph-Rot) on week 10.
A, The ratio of currents elicited by an EC50 concentration of ACh and 100 µm menthol over the current elicited by ACh alone. A, The ratio of currents elicited.
MSCaTs measured by GCaMP6f imaging reflect NMDAR activation at individual synapses following spontaneous single vesicle release. mSCaTs measured by GCaMP6f.
Synaptic depression in principal neurons of the rat MNTB
Depression of NMDA receptor-mediated EPSCs
Differences in MT cell responses between fast and slow sniff cycles.
Removal of the PNNs affect LFP oscillations during spontaneous activity, but not during stimulus-evoked activity. Removal of the PNNs affect LFP oscillations.
Electrode position and representative waveforms of a presumed MSNs and CPNs. A, Electrode position was verified through histological analysis; the coronal.
Sensorimotor cortex recordings have a canonical shape.
Test of visual performance, cortical activity maps and residual S-cone density of the same animal, a ChR2rd1/rd1 mouse, 223 d old. Test of visual performance,
Presentation transcript:

Recording CAPs from mouse optic nerve. Recording CAPs from mouse optic nerve. A, Set-up diagram, the signal is generated with a stimulator and conveyed into the optic nerve through a suction electrode, then recorded using a second suction electrode and send to the amplifier. At the same time, the recording chamber is perfused with Locke solution, which recirculates to get aerated in carbogen every turn. O.N., optic nerve. B, Time line of experiment. The time was set at 0 once the ε-toxin is added to the recording chamber. Three train stimulations of 100 Hz that lasted for 200 ms were applied to the optic nerve: one before the addition of the ε-toxin (t-5), 50 min later (t50), and a last, 80 min (t80). Before ε-toxin addition, the optic nerve is let 30 min to stabilize to the set-up and Locke solution. C, Settings for CAPs analysis. The axes were placed to calculate individually the amplitude and area of the CAP. C1 and C2 are the axes set at the beginning and at the end of the CAP, respectively. The red line is set at the base level. l determines the maximum amplitude of the CAP. D, CAPs elicited at low-frequency stimulation (0.03 Hz) in control conditions before and after adding 50 µl of PBS (vehicle). At -5 min (black), minute 50 (blue), and 85 min (red). E, In ε-toxin condition, examples of CAPs at the same given times. ε-toxin was added dissolved in PBS. Scale bars are represented in each panel. Stimulus artifact was eliminated manually. Differences in shape and amplitudes of initial CAPs in D, E (black) are not related to the action of the ε-toxin, they represent the variability of recording CAPs of different animals. Mercè Cases et al. eNeuro 2017;4:ENEURO.0051-17.2017 ©2017 by Society for Neuroscience