Metformin (Met) reduces membrane-associated TRPA1 levels in rat DRG neurons. Metformin (Met) reduces membrane-associated TRPA1 levels in rat DRG neurons.

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Metformin (Met) reduces membrane-associated TRPA1 levels in rat DRG neurons. Metformin (Met) reduces membrane-associated TRPA1 levels in rat DRG neurons. Immunofluorescent staining was used to detect membrane TRPA1 expression in rat DRG neurons. Representative images acquired using a confocal microscope showing rat DRG neurons under bright field (A and B) and the corresponding images with membrane TRPA1 immunofluorescence (C and D, respectively). Membrane TRPA1 expression was lower in rat DRG neurons treated with Met (500 μmol/L) for 2.5 min (D) than in nontreated DRG neurons (control [con]; C). Black and white arrowheads indicate analyzed and nonanalyzed neurons, respectively (see research design and methods). E: Individual neuron profiles plotted from cross-sectional intensity. Black dots: intensity of neurons not treated with Met (187 neurons); red dots: intensity of neurons treated with Met (206 neurons). F: Box plot showing intensity of con- and Met-treated neurons (n = 187–206). Box borders indicate the 25th and 75th percentiles of the predictor variable. The horizontal line shows the median in the box, and black dots indicate outliers. Numbers in parentheses: numbers of cells tested in each group. ***P < 0.001, unpaired t test. Shenglan Wang et al. Diabetes 2018;67:98-109 ©2018 by American Diabetes Association