Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders Annick A.J.M.

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Presentation transcript:

Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders Annick A.J.M. van de Ven, MD, Ewoud B. Compeer, MSc, Andries C. Bloem, PhD, Lisette van de Corput, PhD, Marielle van Gijn, PhD, Joris M. van Montfrans, MD, PhD, Marianne Boes, PhD Journal of Allergy and Clinical Immunology Volume 129, Issue 3, Pages 755-761.e7 (March 2012) DOI: 10.1016/j.jaci.2011.10.020 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Decreased calcium mobilization in B lymphocytes from patients with CVID disorders. A, Ca2+ mobilization kinetics (left), AUCs (middle), and ratios (right). Cutoff levels are at a level above which 90% of the healthy control subjects have positive results. B, B-cell differentiation correlated with Ca2+ mobilization in patients with CVID disorders. C, Percentages (left) and absolute numbers (right) of IgG+ memory B cells correlated with BCR-mediated calcium influx in age-corrected CVID disorders (n = 30). Pearson correlations: ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Plasma membrane calcium-channel function in B cells from patients with CVID disorders. A, ER Ca2+ release on anti-IgM in calcium-depleted medium: averaged kinetics plot (left) and Ca2+ ratios (right) in B cells of 11 control subjects and 6 patients with CVID disorders. B, CaCl2 addition induces Ca2+ influx in LCLs from patients and control subjects (both n = 12). Lines indicate medians. Mann-Whitney U test: ∗P < .05. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Anti-CD20 induces calcium influx and BCR/coreceptor internalization. A, Colocalization of IgM (green) and CD20 (red) in resting B cells. B, Anti-CD20 triggers a specific Ca2+ flux in B lymphocytes from healthy subjects (left) and patients with CVID disorders (right). C, Anti-CD20 treatment of healthy B cells leads to selective internalization of molecules clustered in the BCR/coreceptor complex. Paired t test: ∗P < .05. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Anti-CD20 does not exhaust BCR-mediated calcium mobilization in patients with CVID disorders. A and B, Anti-CD20 pretreatment decreases Ca2+ mobilization (Fig 4, A) and CD19 surface expression (Fig 4, B) in primary B cells of 10 control subjects and 8 patients (means ± SEMs). Paired t test: ∗P < .05. C, IgM BCR (green) and CD20 (red) dissociate on anti-IgM F(ab′)2 stimulation of LCLs from control subjects and patients with CVID disorders. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Characteristics of patients with CVID disorders with disease-related complications. A, Autoreactive CD21−CD38− B-cell numbers are increased in patients with CVID with autoimmune complications (right) compared with numbers seen in patients without autoimmunity (left). B, Patients with CVID disorders with autoimmune or lymphoproliferative complications had greater calcium mobilization than patients without these complications. Kruskal-Wallis with post hoc Dunn comparisons: ∗∗∗P < .001. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Expression of B-cell surface molecules in patients with CVID disorders. A, CD19 expression in 5 adults, 10 healthy children, and 34 children with CVID disorders. B, Mean fluorescence intensity (MFI) of IgM on naive B cells and memory B cells. C and D, CD20 (Fig E2, C) and CD22 (Fig E2, D) expression in patients and control subjects. Kruskal-Wallis with post hoc Dunn comparisons: ∗P < .05. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 BCR-mediated upregulation of costimulatory molecules. Percentages of CD80+ (A) and CD86+ (B) B cells after 24 hours of culture. Means ± SEMs of 16 control subjects and 7 patients. Mann-Whitney U test: ∗P < .05. C, NFAT gene transcription in PBMCs of 4 control subjects and 3 patients after 48 hours of stimulation with anti-IgM F(ab′)2 fragments. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 ER calcium mobilization and storage capacity in B lymphocytes from patients with CVID disorders. Kinetics of ER storage Ca2+ release after ionomycin stimulation (A) or thapsigargin depletion (B) in calcium-depleted medium, as demonstrated by an averaged kinetics plot (left) and AUC (right) in LCLs from 6 control subjects and 6 patients with CVID disorders. Bar graphs represent means ± SEMs. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E5 Impaired BCR-induced BCR/CD20 lateral dissociation in patients with CVID disorders. A, Colocalization was calculated by dividing double-positive pixels (BCR/CD20) by the positive pixels within the BCR image. B, LCLs from control subjects (left) and patients with CVID disorders (right) before (upper panel) and after (lower panel) BCR stimulation. C, Percentage of dissociation on BCR triggering based on greater than 25 cells per donor in 2 experiments. Journal of Allergy and Clinical Immunology 2012 129, 755-761.e7DOI: (10.1016/j.jaci.2011.10.020) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions