Client-induced retrieval of ERp44.

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Client-induced retrieval of ERp44. Client-induced retrieval of ERp44. (A) Sumf1 can also bind ERp44 after O-glycosylation. HeLa cells were co-transfected as indicated. Aliquots from the lysates were then resolved by SDS-PAGE and blots stained with anti-HA. Note the accumulation of non-glycosylated histidine mutants in the lysates of cells co-expressing Ero1α or Prx4. Instead, Sumf1 expression allows significant accumulation of O-glycosylated ABCD ERp44. (B) Sequential client-induced retrieval of ERp44 histidine mutants. The histograms show the ratio between modified and unmodified ABCD ERp44 in the presence of Ero1α, Prx4, Sumf1, Ig-μΔCH1 or adiponectin (APN). Data represent the mean±s.d. of three or more experiments. (C) Endogenous ERp44 is O-glycosylated in primary endometrial cells. Aliquots of the lysates obtained from endometrial cells in their proliferative (lanes 4–5) or secretory (lanes 3, 6–7) phase (Di Blasio et al., 1995) were digested with (lanes 5, 7) or without (lanes 3, 4, 6) O-glycosidase and resolved by gel electrophoresis under reducing conditions. Note that upon digestion, the doublet visible in untreated samples collapses into a single higher mobility band, which comprises un-glycosylated and de-glycosylated ERp44 (green arrow). The black arrow points at the O-glycosylated species, the main form accumulating in HeLa (lane 2) or endometrial (lane 3) cells after treatment with BFA. (D) Endogenous ERp44 is secreted by endometrial stromal cells. Aliquots from the lysates or spent media (48 h) corresponding to 8×104 or 7×105 endometrial cells in their secretory phase were immunoprecipitated (IP) with the 36C9 monoclonal anti-ERp44 antibody, resolved under reducing conditions and stained with rabbit anti-ERp44 antibody (JDA1). Lanes 1 and 2 show one-third of the spent medium before (Input) or after (L.O., left over) immunoprecipitation. The band migrating above the 50-kDa marker is BSA, which is highly abundant in fetal calf serum. Cross-linked beads were used as a negative control (lane 4). Endogenous ERp44 is clearly detectable in the culture medium and migrates with a molecular mass similar to the O-glycosylated ERp44 species present intracellularly (black arrow). Sara Sannino et al. J Cell Sci 2014;127:4260-4269 © 2014. Published by The Company of Biologists Ltd