A general strategy for the generation of hypoallergenic molecules for the immunotherapy of fish allergy Ines Swoboda, PhD, Nadja Balic, Christoph Klug,

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Presentation transcript:

A general strategy for the generation of hypoallergenic molecules for the immunotherapy of fish allergy Ines Swoboda, PhD, Nadja Balic, Christoph Klug, MSc, Margit Focke, PhD, Milena Weber, MSc, Susanne Spitzauer, MD, Angela Neubauer, PhD, Santiago Quirce, MD, Nikolaos Douladiris, MD, Nikolaos G. Papadopoulos, MD, Rudolf Valenta, MD Journal of Allergy and Clinical Immunology Volume 132, Issue 4, Pages 979-981.e1 (October 2013) DOI: 10.1016/j.jaci.2013.04.027 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, Position of the conserved aspartic acids in the 2 calcium-binding regions in fish parvalbumins and their exchange to alanines in the mutant proteins. B, Sequences of the 2 calcium-binding domains of wild-type and mutated parvalbumins Cyp c 1 and Cyp c 1 Mut (carp), Sal s 1 and Sal s 1 Mut (Atlantic salmon), Thu a 1 and Thu a 1 Mut (tuna), Sco j 1 and Sco j 1 Mut (chub mackerel), Clu h 1 and Clu h 1 Mut (herring), and Gad c 1 and Gad c 1 Mut (codfish). Journal of Allergy and Clinical Immunology 2013 132, 979-981.e1DOI: (10.1016/j.jaci.2013.04.027) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE reactivity and reactivity to antiparvalbumin mutant antibodies of the wild-type and mutant proteins assessed by immunoblot analyses. Wild-type and mutated parvalbumins from Atlantic salmon (Sal s 1, Sal s 1 Mut), tuna (Thu a 1, Thu a 1 Mut), chub mackerel (Sco j 1, Sco j 1 Mut), herring (Clu h 1, Clu h 1 Mut), codfish (Gad c 1, Gad c 1 Mut), and carp (Cyp c 1, Cyp c 1 Mut) were exposed to anticarp parvalbumin mutant mouse antibodies (A) and to serum IgE from fish-allergic patients and 1 nonallergic individual (B). Arrows mark parvalbumin monomers and dimers. Journal of Allergy and Clinical Immunology 2013 132, 979-981.e1DOI: (10.1016/j.jaci.2013.04.027) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions