Thrombogenesis of different cell types seeded on vascular grafts and studied under blood-flow conditions Paul Ph.A.Hedeman Joosten, MD, Hence J.M. Verhagen,

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Presentation transcript:

Thrombogenesis of different cell types seeded on vascular grafts and studied under blood-flow conditions Paul Ph.A.Hedeman Joosten, MD, Hence J.M. Verhagen, MD, PhD, Glenda J. Heijnen-Snyder, Theo J.M.V. van Vroonhoven, MD, PhD, Jan J. Sixma, MD, PhD, Phillip G. de Groot, PhD, Bert C. Eikelboom, MD, PhD Journal of Vascular Surgery Volume 28, Issue 6, Pages 1094-1103 (December 1998) DOI: 10.1016/S0741-5214(98)70036-9 Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Diagram of seeding/perfusion chamber. I, Large caps on which pieces of ePTFE prostheses (II) were immobilized. The rectangular chamber (III) was placed over these 2 caps, which exposed 10 mm × 18 mm of graft surface to flow. The seeding caps (IV) or perfusion knobs (V) could be placed in upper side of chamber. Journal of Vascular Surgery 1998 28, 1094-1103DOI: (10.1016/S0741-5214(98)70036-9) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Diagram of flow circuit that was used for recirculation perfusions. Journal of Vascular Surgery 1998 28, 1094-1103DOI: (10.1016/S0741-5214(98)70036-9) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Ex vivo perfusion. Non-anticoagulated blood is drawn from antecubital vein through perfusion chamber. Blood samples are taken through tubing. After the experiment, perfused graft will be taken out of perfusion chamber and fixed for scanning. Journal of Vascular Surgery 1998 28, 1094-1103DOI: (10.1016/S0741-5214(98)70036-9) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Surfaces of ePTFE graft perfused for 5 minutes with non-anticoagulated blood (shear rate 100/s). A, ePTFE graft seeded with MVECs demonstrates almost no fibrin generation and minimal thrombocyte aggregation. B, Standard plain ePTFE graft shows fibrin formation and thrombocyte aggregation. Journal of Vascular Surgery 1998 28, 1094-1103DOI: (10.1016/S0741-5214(98)70036-9) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions