EBOLA Ag K-SeT rapid test: field evaluation in Sierra Leone F. Colavita, M. Biava, P. Mertens, Q. Gilleman, C. Borlon, M. Delli Guanti, A. Petrocelli, G. Cataldi, A.T. Kamara, S.A. Kamara, K. Konneh, D. Vincenti, C. Castilletti, S. Abdurahman, A. Mirazimi, M.R. Capobianchi, G. Ippolito, R. Miccio, A. Di Caro  Clinical Microbiology and Infection  Volume 24, Issue 6, Pages 653-657 (June 2018) DOI: 10.1016/j.cmi.2017.10.019 Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 EBOLA Ag K-SeT, lateral flow immunoassay, detecting Ebola virus Zaire strain VP40 viral matrix protein. Rapid antigen test is operated by introduction of 30 μL of plasma or serum, or 50 μL of whole blood, onto centre of sample pad. After inserting four drops (approximately 150 μL) of sample buffer in pad, flow of sample and sample buffer initiates. Visual interpretation of results is performed after 15 minutes' incubation time. Capture of antigen by EBOV VP40–specific antibodies composing test line (T) results in development of red signal. In case of EBOV antigen-negative samples, gold conjugate is captured only by control line, indicating valid result. EBOLA Ag K-SeT showing failed control (C) line are considered invalid. EBOV, Ebola virus. Clinical Microbiology and Infection 2018 24, 653-657DOI: (10.1016/j.cmi.2017.10.019) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Diagnostic algorithm integrating RDT test for management of EVD suspected cases during triage. EVD, Ebola virus disease; RT-PCR, reverse transcription–PCR. Clinical Microbiology and Infection 2018 24, 653-657DOI: (10.1016/j.cmi.2017.10.019) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions