Fig. 1 Synthesis and testing of nIRCats.

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Fig. 1 Synthesis and testing of nIRCats. Synthesis and testing of nIRCats. (A) Schematic of optical catecholamine reporters, nIRCats. Pristine SWNTs are functionalized with (GT)6 oligonucleotides to generate turn-on optical reporters for DA and NE. (B) Fluorescence spectra of nIRCats before (black) and after (red) the addition of 10 μM of DA in an in vitro preparation in phosphate-buffered saline (PBS; without tissue). Multiple emission peaks correspond to unique SWNT chiralities contained within the multichirality mixture. a.u., arbitrary units. (C) Nanosensor optical response to 100 μM DA, NE, GLU, GABA, ACH, serotonin (5-HT), histamine (HIST), octopamine (OCT), and tyramine (TYR) (data from in vitro testing). Black bars represent averages from n = 3 independent measurements, and error bars are calculated as SDs of the n = 3 measurements. Abraham G. Beyene et al. Sci Adv 2019;5:eaaw3108 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).