Hepatic fuel metabolism in male 5αR1-KO and WT mice Hepatic fuel metabolism in male 5αR1-KO and WT mice. 5αR1-KO mice gained more liver weight (A) on a high-fat (HF) diet than their littermate controls (WT), although there were no differences in liver weight when presented as a percentage of body weight (B). Hepatic fuel metabolism in male 5αR1-KO and WT mice. 5αR1-KO mice gained more liver weight (A) on a high-fat (HF) diet than their littermate controls (WT), although there were no differences in liver weight when presented as a percentage of body weight (B). C: Higher absolute liver weight was accompanied by increased hepatic triglyceride (TAG) concentration. Abundance of mRNA measured by quantitative real-time PCR and corrected for the mean of the abundances of three reference genes (Rn18s, Ppia, and Tbp) in liver (D), subcutaneous adipose tissue (E), and mesenteric adipose tissue (F). Changes induced by high-fat diet are shown expressed as a percentage of their genotype control. Black bars denote WT mice, and open bars denote KO mice being fed a control diet; gray bars denote WT mice, and striped bars denote KO mice being fed a high-fat diet. Data are the mean ± SEM, compared by ANOVA followed by post hoc testing with Fisher least significant differences test if appropriate. *P < 0.05 for difference between genotypes within diet; #P < 0.05 for effect of diet within genotype. n = 7–9/group. Gene names and associated proteins are detailed in Supplementary Table 1. Dawn E.W. Livingstone et al. Diabetes 2015;64:447-458 ©2015 by American Diabetes Association