MK-8628 restricts cytokine production in T lymphocytes.

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MK-8628 restricts cytokine production in T lymphocytes. MK-8628 restricts cytokine production in T lymphocytes. (A) Quantitative PCR analysis of Lck, Zap70, Lat, Mapk3, Cd3g, Cd28, Tfrc, and Cd25 transcripts in anti-CD3/CD28 mAb–stimulated CD4+ and CD8+ T lymphocytes treated with 200 nM MK-8628 for 24 h, presented relative to expression in untreated controls. (B and C) Meso Scale Discovery (MSD) analysis of IFN-γ, IL-2, and TNF-α levels in supernatants of anti-CD3/CD28 mAb–stimulated CD4+ (B) and CD8+ (C) T lymphocytes treated with 1 or 0.1 μM or untreated controls after 24 h. (D and E) Intracellular flow cytometry assessment of IFN-γ, IL-2, and TNF-α expression in anti-CD3/CD28 mAb–stimulated CD4+ (D) and CD8+ (E) T lymphocytes treated with 1 or 0.1 μM or untreated controls after 24 h. Data are from one experimental representative of at least three independent experiments and represent triplicate wells (B–E) or from three independent experiments where each symbol represents one of four donors (A); small horizontal red lines indicate the mean. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA with posttest analysis (B–E) or unpaired two-tailed Student t test (A). Peter Georgiev et al. ImmunoHorizons 2019;3:294-305 Copyright © 2019 The Authors