AtG3BP1 is associated with stomatal immunity. AtG3BP1 is associated with stomatal immunity. (A) Stomata closure upon ABA and flg22 treatment. Epidermal peels of 5-week-old WT and Atg3bp1-1 plants were floated in stomata buffer with either 20 μM ABA or 1 μM flg22. Stomatal apertures were evaluated after 1 h. Values represent SEM from three independent experiments. Asterisks indicated significant differences from the WT plants determined by a Mann–Whitney U two-tailed test (P ≤ 0.01). (B, C) Stomata closure upon Pst or Pst COR− inoculation. Epidermal peels of 5-week-old WT and Atg3bp1-1 plants were floated in a suspension of 1 × 108 CFU·ml−1Pst or Pst COR− in stomata buffer. Stomatal apertures were evaluated at the indicated time points. Values represent SEM from three independent experiments. Asterisks indicate significant differences from the WT plants determined by a Mann–Whitney U two-tailed test (P ≤ 0.01). (D) Bacterial titers evaluated 3 h after spray inoculation with 1 × 108 CFU·ml−1Pst or Pst COR− in WT and Atg3bp1-1 plants. Values represent SEM from three independent experiments. Asterisks indicate significant differences from the WT plants as determined by a Mann–Whitney U two-tailed test (P ≤ 0.05). (E) Stomata density in leaf abaxial surfaces of WT and Atg3bp1-1 (D, stomata per mm2). (F). Average stomatal size in leaf abaxial surfaces of WT and Atg3bp1-1. Mean pore depth (l mm), which is assumed to be equivalent to guard cell width. (G) Bacterial titers evaluated 3 and 72 h after spray inoculation with 1 × 108 CFU·ml−1Pst in WT and Atg3bp1-1. Values represent SEM from three independent experiments. Asterisks indicate significant differences from the WT plants as determined by a Mann–Whitney U two-tailed test (P ≤ 0.01). (H) Bacterial titers evaluated 3 and 48 h after infiltration inoculation with 1 × 108 CFU·ml−1Pst in WT and Atg3bp1-1 plants. Values represent SEM from three independent experiments. Asterisks indicate significant differences from the WT plants as determined by a Mann–Whitney U two-tailed test (P ≤ 0.01). Aala A Abulfaraj et al. LSA 2018;1:e201800046 © 2018 Abulfaraj et al.