Conservation of key sequence features and endocytic localisation in TbCALM. Conservation of key sequence features and endocytic localisation in TbCALM.

Slides:

Advertisements

Similar presentations

Conservation of Structure and Mechanism between Eukaryotic Topoisomerase I and Site-Specific Recombinases Chonghui Cheng, Paul Kussie, Nikola Pavletich,

Advertisements

Multiple sequence alignment and analysis of SOFL proteins.

Hierarchical Binding of Cofactors to the AAA ATPase p97

Volume 124, Issue 1, Pages (January 2006)

Alignment of H-NS, H-NS2, and StpA amino acid sequences.

Volume 20, Issue 10, Pages (September 2017)

Volume 15, Issue 1, Pages (January 2007)

Volume 19, Issue 4, Pages (April 2011)

Yuji Chikashige, Yasushi Hiraoka Current Biology

Structural and Functional Studies of the 252 kDa Nucleoporin ELYS Reveal Distinct Roles for Its Three Tethered Domains Silvija Bilokapic, Thomas U. Schwartz

Volume 124, Issue 5, Pages (March 2006)

Volume 37, Issue 2, Pages (January 2010)

Volume 5, Issue 3, Pages (September 2003)

Volume 29, Issue 6, Pages (March 2008)

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

Multiple sequence alignment of STAT6 and other STAT proteins produced by ClusterW and ESpript (espript.ibcp.fr/ESPript/ESPript/). Multiple sequence alignment.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

The distribution of lamin C and emerin in lymphoblastoid cell lines.

The C-terminal membrane-proximal region of MARCH8 interacts with TfR.

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Indirect fluorescence assay of N. gruberi cells.

PfSec13 is a structural homolog of ScSec13·Nup145C.

Proteins associated with PfSec13.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

LIR motif consensus and structural determinants of LIR–ATG8 interactions. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A)

Annoted amino acid sequence of Aedes aegypti gliotactin (Gli).

Mosquito GluCl alignment and anti-AgGluCl IgG specificity.

Reconstitution of GBF1 recruitment to Golgi membranes in a cell-free assay. Reconstitution of GBF1 recruitment to Golgi membranes in a cell-free assay.

Comparative genomic expressive hybridisation detection and quantification. Comparative genomic expressive hybridisation detection and quantification. (A)

Example of a putative bridging fiber.

Colocalisation and influence on migration.

Ezrin in its open, activated conformation binds and colocalizes with MISP at the cell cortex. Ezrin in its open, activated conformation binds and colocalizes.

Alignment of the deduced amino acid sequences of the myosin light chain 2 (MLC2) proteins. Alignment of the deduced amino acid sequences of the myosin.

Facilitated migration of implanted Schwann cells infected with Ad-Cdc2 viral vectors in the injured sciatic nerve. Facilitated migration of implanted Schwann.

Two domains of KHC act to position the nucleus.

Fig. 2. RGD and KGD motifs in N. vectensis thrombospondins

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

HADAT1 is a nucleocytoplasmic shuttling protein that is excluded from the nucleolus. hADAT1 is a nucleocytoplasmic shuttling protein that is excluded from.

RAD51 interacts with SUMO-1 through its SIM

KIF13A overexpression favors NP distribution to the cell edge and requires KIF13A binding to microtubules. KIF13A overexpressionfavorsNP distribution to.

Wrch-1 localizes to focal adhesions.

LC8 and its binding partners display broad cellular localization.

Depletion of EB1 with small interfering RNA reduces MT minus-end anchoring at the centrosome. Depletion of EB1 with small interfering RNA reduces MT minus-end.

PtdIns(4,5)P2 dependence of TbEpsinR and TbCALM membrane targeting.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Proliferative and morphological defects following TbCALM and TbEpsinR depletion. Proliferative and morphological defects following TbCALM and TbEpsinR.

Effects of paclitaxel and vinblastine.

Ub-binding ability of ERα.

Dynamics of actin-binding proteins in the ICAM-1 complex.

Lysosomal duplication and segregation defects after TbCALM depletion.

Dynamic and polarized recruitment of ERC1a near protrusive sites during migration. Dynamic and polarized recruitment of ERC1a near protrusive sites during.

Telomere cohesion is established in S phase in cohesin-ring-depleted cells. Telomere cohesion is established in S phase in cohesin-ring-depleted cells.

Subnuclear localization of HA-tagged EBNA1 protein.

TbERP1, TbERP2, TbERP4, and TbERP8 localize to ERES in PCF cells.

Structure–function analysis of CGEF-1.

Ezrin phenotypes in the MVID intestine.

Epiplakin binds to keratins via multiple sites.

Nuclear invaginations observed in thin sections are 3D tunnels and crevices, as shown by FIB-SEM and STORM. (A) In thin section TEM, large nuclear invaginations.

DAPK interacts with HSF1 in vivo and in vitro.

Phylogenetic analysis and domain structure of CALM proteins.

Structure of the Histone Acetyltransferase Hat1

Predicted Amino Acid Sequence of the Tomato Cf-4 Protein (Thomas et al

Volume 17, Issue 5, Pages (May 2009)

Crystal Structure of Escherichia coli RNase D, an Exoribonuclease Involved in Structured RNA Processing Yuhong Zuo, Yong Wang, Arun Malhotra Structure

Yuji Chikashige, Yasushi Hiraoka Current Biology

Alignment of the Amino Acid Sequences of NCS and Other PR10/Bet v1 Proteins from Various Plant Species.Deduced amino acid sequences were aligned using.

Presentation transcript:

Conservation of key sequence features and endocytic localisation in TbCALM. Conservation of key sequence features and endocytic localisation in TbCALM. (A) Overview of CALM and AP180 proteins from human (Hs), yeast (Saccharomyces cerevisiae, Sc) and T. brucei. The extent of the ANTH domain is indicated (black box) along with the distribution of putative binding sites for clathrin and adaptors (black and grey bars). Clathrin and adaptor sites are well conserved along with general domain architecture. (B) Multiple sequence alignment of CALM proteins from across eukaryotes. Residues highlighted in dark grey are conserved in over 80% of the included sequences, light grey indicates greater than 60% conservation. Taxa are indicated to the left and horizontal bars above the sequence indicate the positions of secondary structural features (Ford et al., 2001). Residues identified as important for PtdIns(4,5)P2 binding are denoted with asterisks below the sequence. PtdIns(4,5)P2-binding residues are well conserved. (C) Immunofluorescence localisation of endogenous-locus-tagged TbCALM–GFP in bloodstream form T. brucei. Incubation with ConA at 4°C specifically labels the flagellar pocket (green); anti-GFP staining (red) shows TbCALM–GFP colocalised with the flagellar pocket, consistent with an endocytic function. DNA is stained with DAPI to show the nucleus and kinetoplast (blue). Paul T. Manna et al. J Cell Sci 2015;128:2130-2142 © 2015. Published by The Company of Biologists Ltd