Localization and cellular distribution of pregnancy-associated plasma protein–a and major basic protein in human ovary and corpora lutea throughout the.

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Presentation transcript:

Localization and cellular distribution of pregnancy-associated plasma protein–a and major basic protein in human ovary and corpora lutea throughout the menstrual cycle Alice Rhoton-Vlasak, MD, Gerald J Gleich, MD, Paul Bischof, PhD, Nasser Chegini, PhD Fertility and Sterility Volume 79, Issue 5, Pages 1149-1153 (May 2003) DOI: 10.1016/S0015-0282(03)00077-3

FIGURE 1 Immunohistochemical localization of PAPP-A in human ovarian tissues showing immunostaining in association with (A) primordial follicle, (B–E) mature follicles, and (F) luteal tissue from midluteal phase. Note the immunostaining of theca (TI) and granulosa cell (GC) layers as the size of the follicles increases (B–F) and corpus luteum (CL). TI = theca interna; GC = granulosa cells; CL = corpus luteum. (Magnification: ×61.) Rhoton-Vlasak. MBP and PAPP-A in human ovary. Fertil Steril 2003. Fertility and Sterility 2003 79, 1149-1153DOI: (10.1016/S0015-0282(03)00077-3)

FIGURE 2 Immunohistochemical localization of MBP in human ovarian tissues using polyclonal antibody that recognizes pro-MBP and mature MBP, showing immunostaining in association with (A) primordial follicle, (B and C) mature follicles, and luteal tissue from (D) early luteal phase, (E) midluteal phase, and (F) vasculature (arrow). Note the immunostaining of theca (TE and TI) and granulosa cell (GC) layers as the size of the follicles increases (B and C) and luteal cells. TI = theca interna; TE = theca externa; GC = granulosa cells; LT = luteal tissue. (Magnification: ×61.) Rhoton-Vlasak. MBP and PAPP-A in human ovary. Fertil Steril 2003. Fertility and Sterility 2003 79, 1149-1153DOI: (10.1016/S0015-0282(03)00077-3)

FIGURE 3 Immunohistochemical localization of pro-MBP in human ovarian tissues using monoclonal antibodies showing immunostaining in (A) primordial follicles, (C–D) mature follicles, (E) luteal tissue from midluteal phase, and (F) inflammatory cells found in a corpus albicans. Note the lack of immunostaining of oocytes and stromal cells, and staining of theca and granulosa cell layers as the size of the follicles increases (B–D) and luteal cells. Arrow in E points to a group of small luteal cells. TI = theca interna; TE = theca externa; GC = granulosa cells; LT = luteal tissue; CA = corpus albican. (Magnification: ×61.) Rhoton-Vlasak. MBP and PAPP-A in human ovary. Fertil Steril 2003. Fertility and Sterility 2003 79, 1149-1153DOI: (10.1016/S0015-0282(03)00077-3)