Differential expression of microRNAs in exhaled breath condensates of patients with asthma, patients with chronic obstructive pulmonary disease, and healthy.

Slides:

Advertisements

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Advertisements

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Transient receptor potential vanilloid 1 (TRPV1) antagonism in patients with refractory chronic cough: A double-blind randomized controlled trial Saifudin.

Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.

D-dimer: A biomarker for antihistamine-resistant chronic urticaria

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers Thomas X. Lu,

Exosome-enclosed microRNAs in exhaled breath hold potential for biomarker discovery in patients with pulmonary diseases Anirban Sinha, MSc, Amit Kumar.

Allergen immunotherapy: A practice parameter third update

Risk of an asthma exacerbation after bariatric surgery in adults

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

Alexandra Kazaks, MA, RD, Janet Y. Uriu-Adams, PhD, Judith S

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers Thomas X. Lu,

Is 9 more than 2 also in allergic airway inflammation?

Desensitization to Chemotherapeutic Agents

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

Controlled diesel exhaust and allergen coexposure modulates microRNA and gene expression in humans: Effects on inflammatory lung markers Christopher.

Recurrent anaphylaxis linked to pantoprazole

Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels Rajshekhar Chatterjee, MSc, Jyotsna Batra, PhD, Sudipta.

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Allergy testing in predicting outcome of open food challenge to peanut

Tobacco smoke as a risk factor for allergic sensitization in adults: Conclusions of a systematic review and meta-analysis Beatriz Mónico, MSc, Jorge.

Clinical studies of the DP1 antagonist laropiprant in asthma and allergic rhinitis George Philip, MD, Janet van Adelsberg, MD, Thomas Loeys, PhD, Nancy.

Physician needs in health informatics: Just ask the docs

A network-based analysis of the late-phase reaction of the skin

Peter M. Wolfgram, MD, David B. Allen, MD

The future of allergy/immunology: Promising young investigators

Asthma treatment and asthma prevention: A tale of 2 parallel pathways

An age-dependent association of mannose-binding lectin-2 genetic variants on HIV-1– related disease in children Kumud K. Singh, PhD, Alexis Lieser, MD,

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Staphylococcus aureus colonization is associated with wheeze and asthma among US children and young adults Meghan F. Davis, PhD, Roger D. Peng, PhD,

Food allergy in patients with primary immunodeficiency diseases: Prevalence within the US Immunodeficiency Network (USIDNET) Karen S. Tuano, MD, Jordan.

The brown marmorated stinkbug as a new aeroallergen

Atopic dermatitis: A practice parameter update 2012

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

Daphne Koinis-Mitchell, PhD, Timothy Craig, DO, Cynthia A

News Beyond Our Pages Journal of Allergy and Clinical Immunology

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

Sharon Seth, MD, David A. Khan, MD

What is an “eosinophilic phenotype” of asthma?

H. William Kelly, PharmD Journal of Allergy and Clinical Immunology

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Advances in the approach to the patient with food allergy

Geographic variability in childhood asthma prevalence in Chicago

Mary F. Linehan, PhD, Timothy L. Frank, MD, Michelle L

Alexandra Kazaks, MA, RD, Janet Y. Uriu-Adams, PhD, Judith S

New pathways for itching in patients with atopic dermatitis?

Sputum inflammatory cells from patients with allergic rhinitis and asthma have decreased inflammasome gene expression Willie June Brickey, PhD, Neil.

One step forward, 2 steps back: The enigma of preschool wheeze

E. Rand Sutherland, MD, MPH Journal of Allergy and Clinical Immunology

The impact of a history of asthma on long-term outcomes of people with newly diagnosed chronic obstructive pulmonary disease: A population study Tetyana.

Exosome-enclosed microRNAs in exhaled breath hold potential for biomarker discovery in patients with pulmonary diseases Anirban Sinha, MSc, Amit Kumar.

Measles and immunomodulation

Macrolide antibiotics and asthma treatment

Statistical issues in clinical trials that involve the double-blind, placebo-controlled food challenge Vernon M. Chinchilli, PhD, Laura Fisher, MD, Timothy.

Asthma: The past, future, environment, and costs

Environmental factors and eosinophilic esophagitis

Advances in pediatric asthma in 2007

Safety and efficacy of repeated monthly carboplatin desensitization

Effect of codeine on objective measurement of cough in chronic obstructive pulmonary disease Jaclyn Smith, MD, PhD, Emily Owen, MPhil, John Earis, MD,

Natural history of cow’s milk allergy

Primary prevention of asthma and allergy

Itching as a systemic disease

E. Rand Sutherland, MD, MPH, Barry J

Presentation transcript:

Differential expression of microRNAs in exhaled breath condensates of patients with asthma, patients with chronic obstructive pulmonary disease, and healthy adults Mark Pinkerton, BS, Vernon Chinchilli, PhD, Erin Banta, MD, Timothy Craig, DO, Avery August, PhD, Rebecca Bascom, MD, Margherita Cantorna, PhD, Eric Harvill, PhD, Faoud T. Ishmael, MD, PhD Journal of Allergy and Clinical Immunology Volume 132, Issue 1, Pages 217-219.e2 (July 2013) DOI: 10.1016/j.jaci.2013.03.006 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Box plots showing the expression of specific miRNAs (as RNA copy number/mL of EBC) in patients with asthma, COPD, or healthy controls. *P < .05. Journal of Allergy and Clinical Immunology 2013 132, 217-219.e2DOI: (10.1016/j.jaci.2013.03.006) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 A, Absorbance curve of RNA isolated from EBC. B, A synthetic poly-A tail was added to miRNAs, followed by reverse transcription by using an oligo-dT linked to an adapter. Amplification of a panel of miRNAs by using a specific forward primer and a universal reverse primer to the adapter was analyzed by using qPCR. C, Raw data from qPCR amplification of miRNAs. D, Gel electrophoresis to confirm the expected size of miRNA PCR-products. E, The products of the gel were cloned and sequenced to confirm identity. A representative sequence is depicted for miR-21, showing the miRNA sequence, synthetic poly-A tail, and beginning of the 3′ adapter. qPCR, Quantitative PCR. Journal of Allergy and Clinical Immunology 2013 132, 217-219.e2DOI: (10.1016/j.jaci.2013.03.006) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 A, Expression of small RNAs in EBC that can be used as internal standards (H, healthy; A, asthma; C, COPD). B, Standard curve generated by quantitative PCR of various dilutions of in vitro synthesized Cel-miR-39. Journal of Allergy and Clinical Immunology 2013 132, 217-219.e2DOI: (10.1016/j.jaci.2013.03.006) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions