EV derived from mature BMDC are efficiently taken up by murine monocytes and have a rich content of CCF. EV derived from mature BMDC are efficiently taken.

Slides:

Advertisements

Similar presentations

AB C D E F G H Supplemental Fig. 1. Figure S1. MIF induces maturation of CD11b + but not CD8α + DCs in spleen from Mif -/- mice infected with T. gondii.

Advertisements

Suppl. Fig x106 Culture of Irradiated A-CFSE

Fig. 1. TLR4 expression is much greater on macrophages than on DCs

Figure 3. TLT-6 expressions in hematopoietic stem cells, monocyts and macrophages. (A) Expression patterns of TLT-6 in the surface of hematopoietic lineage.

S2 Fig. Phenotype of donor cells in the spleen and bone marrow of male mice. Splenocytes and bone marrow were isolated from male donor mice and stained.

Effect of pre-treatment with lipopolysaccharide (LPS) or cystic fibrosis (CF) serum on the production of reactive oxygen species (ROS) by phagocytes from.

Supplementary Figure S1: The secondary structure of C1q is irreversibly altered after heat-denaturation. The far UV spectrum was measured by circular dichroism.

Resveratrol modulates DSS-induced neutrophils during colitis.

Christopher L. Kepley, PhD, Bridget S. Wilson, PhD, Janet M

Primary human MNCs and MDMs are targets of ZIKV infection.

Novel function for interleukin-7 in dendritic cell development

Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells by Elke Scandella, Ying Men, Silke Gillessen,

Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization Sophia Björkander, Maria.

The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions by Markus P. Radsak, Norbert Hilf, Harpreet Singh-Jasuja,

A Strong Expression of CD44-6v Correlates With Shorter Survival of Patients With Acute Myeloid Leukemia by S. Legras, U. Günthert, R. Stauder, F. Curt,

Identification in CLL of circulating intraclonal subgroups with varying B-cell receptor expression and function by Vânia Coelho, Sergey Krysov, Andrew.

Volume 45, Issue 2, Pages (August 2016)

IL-22 Increases the Innate Immunity of Tissues

Fig. 1 pDCs infiltrate the skin of SSc patients and spontaneously secrete IFN-α and CXCL4. pDCs infiltrate the skin of SSc patients and spontaneously secrete.

Fig. 2. GPC3 expression in normal and tumor tissues.

Volume 165, Issue 3, Pages (April 2016)

Gr-1+ MDSC in tumor-bearing mice produce IL-6.

Increased chemokine content and leukocyte infiltrate in D6-negative tumors. Increased chemokine content and leukocyte infiltrate in D6-negative tumors.

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Katherine G. MacDonald, BSc, Nicholas A. J

Effect of OPN on apoptosis of DCs

Volume 73, Issue 7, Pages (April 2008)

IL-31 Induces Chemotaxis, Calcium Mobilization, Release of Reactive Oxygen Species, and CCL26 in Eosinophils, Which Are Capable to Release IL-31 Nikola.

DCs induce the proliferation of autologous T cells.

BMDC-EV induce phenotypically different myeloid cell sub-populations.

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

Volume 138, Issue 1, Pages (January 2010)

DC-derived EV differentiate monocytes.

Marker identification and quantification by StrataQuest, and confocal analysis of EV-containing tissue sections. Marker identification and quantification.

The PbGAPDH G6 fragment interacts with recombinant CD68.

DC-EV induce GM-CSF signaling and convey a cornucopia of effector molecules. DC-EV induce GM-CSF signaling and convey a cornucopia of effector molecules.

TLR9 deficiency promotes aberrant T cell and myeloid dendritic cell (DC) phenotype in imiquimod-induced autoimmunity. TLR9 deficiency promotes aberrant.

Defective negative regulation of Toll-like receptor signaling leads to excessive TNF-α in myeloproliferative neoplasm by Hew Yeng Lai, Stefan A. Brooks,

Figure 1 Fingolimod does not alter human monocyte viability Peripheral blood mononuclear cells from healthy donors were briefly exposed to increasing concentrations.

Differential attenuation of β2 integrin–dependent and –independent neutrophil migration by Ly6G ligation by Pierre Cunin, Pui Y. Lee, Edy Kim, Angela B.

BMDC-EV attract immune cells in lymph nodes similarly as in the skin.

Concentrations of CCF in DC-derived EV and DC culture supernatants.

Fig. 4 Surgery initiates a systemic inflammatory response that triggers the outgrowth of distant immunogenic tumors and can be inhibited by perioperative.

Actin assembly in response to thrombin.

ISS forming highly aggregated structures have decreased capability to induce maturation of human PDCs. Purified PDCs (105 cells) were stimulated with ISS.

CpG-C ISS lose their ability to induce IFN-α from human PDCs while retaining the ability to induce maturation when encapsulated into pH 5.75 liposomes.

Differential intracellular localization of ISS in human PDCs

cMΦ develop into 4 subpopulations after birth.

PD-L1 selectively marks circulating NCMs.

CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to.

Senescence-associated defective HLA-DR upregulation does not modulate immunosuppressive properties of MSCs. (A) Fit and senescent MSCs were subjected to.

Thiol Antioxidants Block the Activation of Antigen-Presenting Cells by Contact Sensitizers Stefanie Bruchhausen, Sabine Zahn, Elke Valk, Jürgen Knop,

MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.

Volume 23, Issue 7, Pages (July 2015)

BMS blocks functional responses in primary immune cells driven by IFNα

CT-26 colon cancer induces the recruitment of protumor mast cells and the accumulation of MDSCs. CT-26 colon carcinoma cells (2 × 105) were injected s.c.

Volume 35, Issue 6, Pages (December 2011)

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

Volume 24, Issue 12, Pages (December 2016)

Comparison of OPN production by mature DCs and activated macrophages.

Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Uptake of TEX by DCs in vivo.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Fig. 4 IP6K1-mediated polyP production by platelets plays a critical role in LPS-induced NPA formation. IP6K1-mediated polyP production by platelets plays.

Trametinib and combination promote moDC maturation and decrease moDC viability. Trametinib and combination promote moDC maturation and decrease moDC viability.

Fig. 7 Differences in the tumor microenvironment between transplant and transgenic BRAFV600E-driven melanoma models may underlie refractoriness of iBIP2.

Lack of innate immune cell and receptor triggering by Adjuplex.

Surface expression of chemokine receptors on M

Presentation transcript:

EV derived from mature BMDC are efficiently taken up by murine monocytes and have a rich content of CCF. EV derived from mature BMDC are efficiently taken up by murine monocytes and have a rich content of CCF. (A) Murine PBMCs were incubated with stained (PKH26) immature (im) or mature (ma) BMDC-EV for 2 h. Percent of PKH26+ cells was determined by flow cytometry gating on monocytes (Ly6C-high, CD11b+, and Ly6G−), B cells (B220+, CD11b−, Ly6C−, and Ly6G−), and granulocytes (SSC-high, Ly6G+, CD11b+, Ly6C-int., and B220−). (B) EV derived from matured BMDC contain a rich CCF. EV derived from mature BMDC, which had been stimulated with Poly IC/R848 (EV-BMDC + Poly I:C) or LPS (BMDC-EV + LPS) or unstimulated (BMDC-EV), were analyzed by an antibody-based bead array (BioLegend). Values were normalized to number of EV-producing cells. Five different donors were analyzed. The resulting numbers were used to calculate mean values ± SEM. (C) Lysates from EV as described in (B), but derived from cells of a different donor animal, were analyzed by a protein array as described in Fig 3. The pixel intensity of each dot was determined by ImageJ, and the value was adjusted in relation to the internal positive control, which was set to 1. Shown is one representative analysis performed with four different donors. Stefan Schierer et al. LSA 2018;1:e201800093 © 2018 Schierer et al.