Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

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Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP.Schneider cells were transfected with GFP-dFMRP and its mutant versions. Analysis of dFMRP kinetics in dFMRP granules by FRAP.Schneider cells were transfected with GFP-dFMRP and its mutant versions. (A–C) 48 h post transfection, a single dFMRP granule (red circle; indicated by arrow) was photobleached and fluorescence recovery was recorded over 140 s using a confocal microscope. The FRAP methodology is described in Materials and Methods. Scale bar: 10 µm. The images shown in (A) were selected for illustration and correspond to merged differential interference contrast (DIC) microscopy and fluorescence pictures. The recovery of dFMRP fluorescence in the photobleached area was quantified as described in Materials and Methods and plotted as a function of time as indicated in (B). Curves are representative of 3 independent experiments with a total of 100 photobleached granules for each indicated GFP fusion protein. (C) Bar graphs for the MF of each GFP fusion protein are shown with error bars corresponding to the SD of 3 independent experiments. The indicated P-values are calculated with unpaired Student's t-test (n = 3). Cristina Gareau et al. Biology Open 2013;2:68-81 © 2012. Published by The Company of Biologists Ltd