Monoclonal antibody production and application

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Monoclonal antibody production and application Monoclonal antibody production and application Immunization leads to B-cells producing antibodies that bind to an antigen of interest. Individual B-cells are fused with partners that immortalize them and the resulting B-cell hybridomas produce identical (monoclonal) antibodies indefinitely. Following screening, relevant hybridomas are selected and expanded to produce large quantities of uniform antibody-producing cells. Such antibodies have many applications, for example locating antigen in tissue samples, providing the basis of a specific test for pregnancy hormone or being genetically engineered to use in immunotherapy of disease. Pregnancy test: Nabokov, en.wikipedia.org/w/index.php?curid=36047325 Lindsay B. Nicholson Essays Biochem. 2016;60:275-301 ©2016 by Portland Press Ltd