Helicobacter pylori and Gastric Cancer: A New Paradigm For Inflammation-Associated Epithelial Cancers  JeanMarie Houghton, Timothy C. Wang  Gastroenterology  Volume 128, Issue 6, Pages 1567-1578 (May 2005) DOI: 10.1053/j.gastro.2005.03.037 Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 1 Multistep model for the progression of gastric cancer (adapted from Fox JG, Wang TC. N Engl J Med 2001;345:829–832). Gastroenterology 2005 128, 1567-1578DOI: (10.1053/j.gastro.2005.03.037) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 2 Engraftment of bone marrow-derived cells in the gastric mucosa: effects of time and infection. C57BL/6 mice were myloablated and underwent transplantation with marrow from wild-type C57BL/6 or ROSA-26 mice carrying the β-galactosidase gene. After recovery, mice were infected with Helicobacter felis or vehicle control. Engrafting cells were detected using x-gal reagent, which produces a blue cytoplasm in cells expressing the β-galactosidase enzyme. (A) C57BL/6 mice that underwent transplantation with C57BL/6 marrow, infected with H felis for 30 weeks, do not show reactivity with x-gal reagent (blue). Larger area from the same mouse shown in F. (B) C57BL/6 mice that underwent transplantation with ROSA-26 marrow and sham infected or, in C, infected with H felis for 4 weeks demonstrate marrow-derived (blue) inflammatory cells. We did not demonstrate any gastric epithelial glands, which were bone marrow derived. (D) ROSA transplantation mice infected for 20 weeks had few marrow-derived glands (blue, arrow). (E) By 12–18 months of chronic infection, large numbers of glands were completely replaced by cells that were marrow derived (blue). (G) Low-power view of the area. (Figure provided courtesy of Dr JeanMarie Houghton.) Gastroenterology 2005 128, 1567-1578DOI: (10.1053/j.gastro.2005.03.037) Copyright © 2005 American Gastroenterological Association Terms and Conditions

Figure 3 The new model of epithelial cancer. (Figure provided courtesy of Dr JeanMarie Houghton.) Gastroenterology 2005 128, 1567-1578DOI: (10.1053/j.gastro.2005.03.037) Copyright © 2005 American Gastroenterological Association Terms and Conditions