Matrix metalloproteinase inhibitor regulates inflammatory cell migration by reducing ICAM-1 and VCAM-1 expression in a murine model of toluene diisocyanate-induced.

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Matrix metalloproteinase inhibitor regulates inflammatory cell migration by reducing ICAM-1 and VCAM-1 expression in a murine model of toluene diisocyanate-induced asthma Kyung S. Lee, MS, Sun M. Jin, BS, Hyung J. Kim, MD, PhD, Yong C. Lee, MD, PhD Journal of Allergy and Clinical Immunology Volume 111, Issue 6, Pages 1278-1284 (June 2003) DOI: 10.1067/mai.2003.1501 Copyright © 2003 Mosby, Inc. Terms and Conditions

Fig. 1 Effect of TDI challenge on ICAM-1, VCAM-1, and MMP-9 expression in lung tissues and in BAL fluids. A, C, and E, RT-PCR of ICAM-1, VCAM-1, and MMP-9. Densitometric analyses are presented as relative ratio of each molecule mRNA to GAPDH mRNA. Relative ratio of each molecule mRNA in lung tissues of control mice (Co) is arbitrarily presented as 1. B, D, and F, Western blotting of ICAM-1, VCAM-1, and MMP-9. Densitometric analyses are presented as relative ratio of each protein to actin. Relative ratio of each protein in lung tissues of control mice is arbitrarily presented as 1. G, Gelatin zymography. Densitometric analyses are presented as relative ratio of induction of pro-MMP-9 before and after TDI challenge. Pro-MMP-9 in BAL fluids of control mice is arbitrarily presented as 1. H, Enzyme immunoassay of pro–MMP-9. Data represent mean ± SD from 6 independent experiments. EO, Vehicle control; Co, no treatment; Pre, before challenge; 1, 6, 24, 48 and 72 hours, after challenge. *P < .05 vs Pre; #P < .05 vs EO. Journal of Allergy and Clinical Immunology 2003 111, 1278-1284DOI: (10.1067/mai.2003.1501) Copyright © 2003 Mosby, Inc. Terms and Conditions

Fig. 2 Effect of MMPI-I or MMPI-II on total and differential cellular components of BAL fluids in TDI-sensitized and TDI-challenged mice. Sampling was performed at 72 hours after treatment with vehicle only (EO) , EO plus TDI, EO plus TDI plus DMSO, EO plus TDI plus MMPI-I, or EO plus TDI plus MMPI-II. Numbers of each cellular component were counted. Bars represent mean ± SD from 6 independent experiments. TC, Total cell; MAC, macrophage; LYM, lymphocyte; NEU, neutrophil; EOS, eosinophil. *P < .05 vs EO; #P < .05 vs EO+TDI. Journal of Allergy and Clinical Immunology 2003 111, 1278-1284DOI: (10.1067/mai.2003.1501) Copyright © 2003 Mosby, Inc. Terms and Conditions

Fig. 3 Effect of MMPI-I or MMPI-II on ICAM-1 and VCAM-1 expression in lung tissues of TDI-sensitized and TDI-challenged mice. Sampling was performed at 72 hours after treatment with vehicle only (EO) , EO plus TDI (ET) , EO plus TDI plus DMSO (ETD) , EO plus TDI plus MMPI-I (ETMI) , or EO plus TDI plus MMPI-II (ETMII) . A, RT-PCR of ICAM-1 and VCAM-1. Densitometric analyses are presented as relative ratio of ICAM-1 or VCAM-1 mRNA to GAPDH mRNA. Relative ratio of ICAM-1 or VCAM-1 mRNA in lung tissues of EO mice is arbitrarily presented as 1. B and C, Western blotting of ICAM-1 and VCAM-1. Densitometric analyses are presented as relative ratio of ICAM-1 or VCAM-1 to actin. Relative ratio of ICAM-1 or VCAM-1 in lung tissues of EO mice is arbitrarily presented as 1. Data represent mean ± SD from 6 independent experiments. *P < .05 vs EO; #P < .05 vs ET. Journal of Allergy and Clinical Immunology 2003 111, 1278-1284DOI: (10.1067/mai.2003.1501) Copyright © 2003 Mosby, Inc. Terms and Conditions

Fig. 4 Effect of MMPI-I or MMPI-II on IL-1β, IL-4, and TNF-α mRNA expression in lung tissues and IL-1β, IL-4, and TNF-α levels in BAL fluids of TDI-sensitized and TDI-challenged mice. Sampling was performed at 72 hours after treatment with vehicle only (EO) , EO plus TDI (ET) , EO plus TDI plus DMSO (ETD) , EO plus TDI plus MMPI-I (ETMI) , or EO plus TDI plus MMPI-II (ETMII) . A, C, and E, RT-PCR of IL-1β, IL-4, and TNF-α. B, D, and F, Densitometric analyses are presented as relative ratio of each molecule mRNA to GAPDH mRNA. Relative ratio of each molecule mRNA in lung tissues of EO mice is arbitrarily presented as 1. G, Enzyme immunoassay of IL-1β, IL-4, and TNF-α. Data represent mean ± SD from 6 independent experiments. *P < .05 vs EO; #P < .05 vs ET. Journal of Allergy and Clinical Immunology 2003 111, 1278-1284DOI: (10.1067/mai.2003.1501) Copyright © 2003 Mosby, Inc. Terms and Conditions