Heat denaturation affects the ProDer p 1 IgE reactivity and downregulates the development of the specific allergic response Mauro Magi, MD, Lida Garcia,

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Presentation transcript:

Heat denaturation affects the ProDer p 1 IgE reactivity and downregulates the development of the specific allergic response Mauro Magi, MD, Lida Garcia, MD, Michel Vandenbranden, PhD, Rémi Palmantier, PhD, Alain Jacquet, PhD Journal of Allergy and Clinical Immunology Volume 114, Issue 3, Pages 545-552 (September 2004) DOI: 10.1016/j.jaci.2004.04.014

Fig 1 Experimental protocol for the evaluation of the immune responses in naive and allergic mice. Journal of Allergy and Clinical Immunology 2004 114, 545-552DOI: (10.1016/j.jaci.2004.04.014)

Fig 2 Effect of heat denaturation on the IgE reactivity of recombinant ProDer p 1. The IgE reactivity of natural Der p 1 and different preparations of recombinant ProDer p 1 were evaluated in direct ELISA and by using radioallergosorbent positive sera to D pteronyssinus. Journal of Allergy and Clinical Immunology 2004 114, 545-552DOI: (10.1016/j.jaci.2004.04.014)

Fig 3 Inhibition of IgE binding to natural Der p 1. IgE binding to immobilized natural Der p 1 was inhibited by preincubation of sera with increasing concentrations of natural Der p 1 (●) and native (■), heat-denatured (○), or reduced, heat-denatured (□) recombinant ProDer p 1. Results are shown as 100% (IgE binding in the presence of inhibitor/IgE binding in the absence of inhibitor). Journal of Allergy and Clinical Immunology 2004 114, 545-552DOI: (10.1016/j.jaci.2004.04.014)

Fig 4 IL-5 and IFN-γ concentrations in supernatants from ProDer p 1–restimulated splenocytes. The production of IL-5 and IFN-γ by ProDer p 1–activated splenic cells from vaccinated naive (A) or allergic (B) mice was measured as described in the section Materials and Methods. Results are expressed as the means ± SDs from 10 mice. Journal of Allergy and Clinical Immunology 2004 114, 545-552DOI: (10.1016/j.jaci.2004.04.014)