Expression of VPAC2 in the pancreas at E13.

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Immunohistochemistry and in situ hybridization allow researchers to pinpoint the expression of their protein and nucleic acid targets, respectively.
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Expression of VPAC2 in the pancreas at E13. Expression of VPAC2 in the pancreas at E13. A: Whole-mount in situ hybridization of dissected gastrointestinal tracts hybridized with a VPAC2 antisense (AS) probe (a), a Pdx1 AS probe (b), or a VPAC2 sense probe (c). B: Gastrointestinal tracts hybridized with a VPAC2 AS probe were sectioned and stained with a Pdx-1 antibody (red). a: VPAC2 expression in the dorsal and ventral pancreas. b: Pdx-1 expression. c: a and b are fused. d: c is enlarged. dp, dorsal pancreas; vp, ventral pancreas; g, gut. Latif Rachdi et al. Diabetes 2003;52:85-92 ©2003 by American Diabetes Association