Effect of the selective pharmacological inhibitors used.

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Effect of the selective pharmacological inhibitors used. Effect of the selective pharmacological inhibitors used. (A) Inhibitory effect of SB203580 on hyperthermia-induced phosphorylation levels of p38-MAPK (top panel) was assessed in samples of gill tissue extracts (50 μg) from control animals (15°C) and animals exposed to hyperthermia (30°C) for 30 min. Equal protein loading was confirmed by blotting identical samples with an anti-actin specific antibody (bottom panel). (B) Inhibitory effect of SP600125 on hyperthermia-induced phosphorylation levels of cJun (top panel) was assessed in the nuclear fractions from gill tissue extracts (30 μg) from control animals (15°C) and animals exposed to hyperthermia (30°C) for 30 min. Equal protein loading was confirmed by blotting identical samples with an anti-histone H1 specific antibody (bottom panel). Bands were quantified by laser scanning densitometry (C, phospho-p38-MAPK and D, phospho-cJun). Blots and results shown are representative of four independent experiments. Results are means ± s.e. for four independent experiments. *P<0.01 compared with identically treated animals in the absence of the respective inhibitor. E. Gourgou et al. J Exp Biol 2010;213:347-357 © 2010.