Csmd2 mRNA is expressed in the mouse forebrain.

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Csmd2 mRNA is expressed in the mouse forebrain. Csmd2 mRNA is expressed in the mouse forebrain. A, Schematic of numbered exons of the mouse Csmd2 gene and domain structure of mouse Csmd2 protein, noting locations of alternative mRNA splicing, probes used for in situ hybridization and quantitative PCR analysis, antigen used to generate the anti-Csmd2 antibody from Novus, and target locations of Csmd2 shRNAs. B, In situ hybridization showed broad expression of Csmd2 mRNA throughout all neuronal layers in the neocortex and hippocampus. A sense-strand probe was used as a negative control. C, Quantitative PCR analysis showed a slight increase in Csmd2 mRNA expression in the neocortex from time point P0 to P7 and P90. Values were normalized to Cyclophilin A expression and graphed (average ± SEM of biological replicates) relative to the P0 time point. A.U., arbitrary units. Mark A. Gutierrez et al. eNeuro 2019;6:ENEURO.0434-18.2019 ©2019 by Society for Neuroscience