97.4 66.2 45 31 21.5 14 MW (kDa) 2 µg 0.5 µg Supplementary Figure S1. SDS-PAGE under reducing condition analysis (Coomassie brilliant blue staining) of.

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97.4 66.2 45 31 21.5 14 MW (kDa) 2 µg 0.5 µg Supplementary Figure S1. SDS-PAGE under reducing condition analysis (Coomassie brilliant blue staining) of human anti-ERα Abs obtained from early ER-positive breast cancer patients. Lanes: 1, molecular weight markers (MW); 2, 2 µg anti-ERα Abs; 3, 0.5 µg anti-ERα Abs.

% of Ki-67-positive cells untreated E2 E2+ anti-ERα Abs E2+ TAM E2+ anti-ERα Abs+ TAM * ** *** * Supplementary Figure S2. Cell proliferation of MCF-7 cells treated with E2 or E2 plus anti-ERα Abs, in presence or absence of tamoxifen. Cell proliferation was evaluated by flow cytometry measuring Ki-67 nuclear antigen expression in MCF-7 cells treated or not for 24 hours with E2 (10 nM), E2 + anti-ERα Abs (50 µg/ml), E2 + TAM 1µM, E2 + anti-ERα Abs + TAM. Data reported as mean ± SD (right), *, P < 0.05; **, P < 0.01; ***, P<0.001, by Student’s t test.