Human IL-10 neutralization results in inhibited expression of intragraft Treg function genes and production of suppressive cytokine IL-10. Human IL-10.

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Human IL-10 neutralization results in inhibited expression of intragraft Treg function genes and production of suppressive cytokine IL-10. Human IL-10 neutralization results in inhibited expression of intragraft Treg function genes and production of suppressive cytokine IL-10. A: Measurement of intragraft mRNA expression of Treg marker and function associated molecules by real-time PCR in mice receiving both human PBMC and Treg and treated with anti–IL-10 mAb (IL-10 mAb day 50) or isotype Ab (Isotype Ab day 100) at days 50 or 100 post-PBMC reconstitution, respectively. Data are presented as mean ± SD of three individual graft samples from one of three independent experiments. B: Intragraft expression of human IL-10 mRNA measured by real-time PCR in the same mice as in A. Data are represented as mean ± SD of three individual graft samples from one of three independent experiments. C: Representative cytokine bead array of measurement of serum IL-10 in the same mice as in A is shown. Open histogram represents isotype control Ab treated, and filled histogram represents anti-human IL-10 mAb treated. D: IL-10 level in serum samples as assessed in C is shown as mean ± SD of three independent mouse samples. *P < 0.05; **P < 0.01, compared with anti–IL-10 mAb-treated mice. Shounan Yi et al. Diabetes 2012;61:1180-1191 ©2012 by American Diabetes Association