Johannes Mathieu, Norman Warthmann, Frank Küttner, Markus Schmid

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Export of FT Protein from Phloem Companion Cells Is Sufficient for Floral Induction in Arabidopsis Johannes Mathieu, Norman Warthmann, Frank Küttner, Markus Schmid Current Biology Volume 17, Issue 12, Pages 1055-1060 (June 2007) DOI: 10.1016/j.cub.2007.05.009 Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 1 Effect of Artificial MicroRNAs against FT and TSF mRNA on Flowering Time in Arabidopsis thaliana Histograms of flowering time of independent T1 lines are shown. Number (n) of independent T1 lines analyzed per transgene is displayed in each panel. Flowering time is given as leaves on the main shoot. Flowering time of amiR-FT lines analyzed in subsequent generations (Table 1) is marked (arrows). Flowering time of Col-0 control plants is indicated (gray box). Current Biology 2007 17, 1055-1060DOI: (10.1016/j.cub.2007.05.009) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 2 Proteolytic Release of FT Protein from an Immobile Precursor (A) Diagrams of FT:TEVrs:3x-YFP fusion proteins with and without nuclear localization signal (NLS). TEV protease recognition site is indicated in bold letters, and the cleavage site is marked with an arrowhead. (B) Protein blot analysis of plants expressing FT:TEVrs:3xYFP:NLS and TEV protease under the control of a SUC2 promoter. Lane 1, Col-0 (wild-type control); lane 2, 3xYFP:NLS; lane 3, TEV protease; lane 4, FT:TEVrs:3xYFP:NLS; lane 5, FT:TEVrs:3xYFP:NLS, TEV protease. Coomassie-stained gel, showing the large subunit of RuBisCO as loading control. Current Biology 2007 17, 1055-1060DOI: (10.1016/j.cub.2007.05.009) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 3 Effect of the Release of FT Protein from Phloem Companion Cells on Flowering (A–F) Col-0 wild-type (A, B), SUC2::TEVP (C, D), and SUC2::FT:TEVrs:3xYFP:NLS (E, F) plants grown in long days (16 hr light; 23°C). YFP fluorescence signal (B, D, F) in rosette leaves (only present in [F]). (G and H) SUC2::TEVP SUC2::FT:TEVrs:3xYFP:NLS doubly transgenic plants flower early (G) and express YFP in the vasculature (H). (I and J) Expression of FT:TEVrs:3xYFP:NLS from the constitutive 35S promoter results in early flowering (I), with YFP detectable throughout the plant (J). Scale bars represent 1 cm. Current Biology 2007 17, 1055-1060DOI: (10.1016/j.cub.2007.05.009) Copyright © 2007 Elsevier Ltd Terms and Conditions