Characterization of the ERα-UBS.

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Characterization of the ERα-UBS. Characterization of the ERα-UBS. (A,C,D) Top: schematic of ERα E domain. Amino acid positions are indicated. Bottom: In vitro pull-down assay using the indicated GST-tagged ERα constructs. GST-fusion proteins were incubated with total cellular lysates extracted from growing HeLa cells (A and C) or with synthetic polyUb2-7 linked by Lys63 (D) and analyzed by immunoblot as indicated. * indicates significant differences with respect to the relative wild-type sample. (B) Top: ClustalW (http://www.ebi.ac.uk/Tools/msa/clustalw2/) alignment of ERα E domain with RNF168 UMI domain (Pinato et al., 2011). Middle: Projected helices using the Helical Wheel Projections software tool (http://rzlab.ucr.edu/scripts/wheel/wheel.cgi). Bottom: Three-dimensional structure of the human ERα LBD-E2 complex (1ERE) (Brzozowski et al., 1997); E2 has been removed. The structure is drawn in green; conserved amino acids between the ERα E domain and RNF168 UMI domain are space-filled and stained as indicated. Valeria Pesiri et al. J Cell Sci 2013;126:2577-2582 © 2013. Published by The Company of Biologists Ltd