Properties of Ca2+ puffs in cells expressing a single IP3R subtype.

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Properties of Ca2+ puffs in cells expressing a single IP3R subtype. Properties of Ca2+ puffs in cells expressing a single IP3R subtype. (A–C) Mean rise time (A) – i.e. time for fluorescence to increase from 20% to 100% of peak, decay time (B) – i.e. time to fall from 100% to 20%, and mean duration at half-maximal amplitude (C) of Ca2+ puffs evoked by photolysis of ci-IP3. Results show mean values for each of 15 cells for each cell line (∼1000 puffs per cell line were analysed), and mean±s.e.m. of these values. *P<0.05, ***P<0.001, relative to WT. Stefania Mataragka, and Colin W. Taylor J Cell Sci 2018;131:jcs220848 © 2018. Published by The Company of Biologists Ltd