Expression of PKCδ in islets and other tissues of control and PKCδKN-overexpressing mice. Expression of PKCδ in islets and other tissues of control and.

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Expression of PKCδ in islets and other tissues of control and PKCδKN-overexpressing mice. Expression of PKCδ in islets and other tissues of control and PKCδKN-overexpressing mice. A: Detection of PKCδ by Western blotting of homogenates of isolated islets from control and PKCδKN mice (#179). B: In islet cells of pancreatic slices that stain positive for insulin (red), a low endogenous and a significant overexpression of PKCδ (green) is detected in control and PKCδKN-overexpressing mice (#179), respectively. C: Western blot analysis of tissue homogenates (50 μg mouse line #179) of control (WT) and PKCδKN-overexpressing (KN) mice reveals a high expression of PKCδ in brain, spleen, and hypothalamus (hypothal) but a low expression of PKCδ in heart, kidney, liver, muscle, and fat. As control, homogenate of INS-1E cells transfected with PKCδWT (15 μg) is blotted on the left. (A high-quality color digital representation of this figure is available in the online issue.)‏ Anita M. Hennige et al. Diabetes 2010;59:119-127 ©2010 by American Diabetes Association