Model of local recycling of the actin network in L

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Cytoskeleton and Cell Motility

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Model of local recycling of the actin network in L Model of local recycling of the actin network in L. monocytogenes protrusions. Model of local recycling of the actin network in L. monocytogenes protrusions. (A) Components of the AIP1-dependent disassembly machinery (CFL1, GMFB, TWF2 and CAP1) whose depletion enhances the spreading defect phenotype displayed by AIP1-depleted cells. (B) The bacterial factor ActA (green dots) promotes the nucleation activity of the ARP2/3 complex (red dots), which leads to the assembly of a branched network at the bacterial pole (blue lines and red dots). As protrusions elongate, the AIP1-dependent disassembly machinery recycles G-actin and ARP2/3 from the distal network, which fuels continuous F-actin assembly at the bacterial pole (red arrow). (C) The life cycle of protrusions can be divided in four phases: (1) Emerging protrusions, actin assembly propels the cytosolic bacterium against the plasma membrane, which protrudes into the adjacent cell; (2) elongating protrusions, as protrusions elongate, the disassembly machinery recycles the distal network thereby fuelling further assembly at the bacterial pole in this confined system; (3) stationary protrusions, as the recycling process exhausts the cytoskeleton components from the distal region of protrusions, protrusions become stationary, and continuous actin assembly results in retrograde flow; and (4) protrusion-to-vacuole transition, complete exhaustion of the cytoskeleton components from the distal network allows for membrane apposition in the distal region of protrusions. Continuous generation of forces due to actin assembly and retrograde flow leads to membrane disruption and resolution of the protrusion into a double-membrane vacuole. Arthur M. Talman et al. J Cell Sci 2014;127:240-249 © 2014. Published by The Company of Biologists Ltd