BCL-3 does not mediate β-catenin activity through promoting nuclear translocation or altering levels of LEF1. BCL-3 does not mediate β-catenin activity.

Slides:



Advertisements
Similar presentations
Control siRNA BRG1 siRNA PTEN – Akt – p-Akt – p-GSK-3  – Cyclin D1 – BRG1 –  -actin – SW480 Supplemental data S1 Supplemental data S1. Effects of transduction.
Advertisements

EGCG prevents PCB-126-induced endothelial cell inflammation via epigenetic modifications of NF-κB target genes in human endothelial cells  Dandan Liu,
E2F7/8 and HIF1 are required for hypoxic VEGFA expression.
Plakoglobin as a Regulator of Desmocollin Gene Expression
Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria  Dan Wang, Chunsun Dai, Yingjian Li, Youhua.
Figure 6 Factors modulating SCN5A gene expression
GLUT3 is a direct target of TCF4/β‐catenin
Volume 138, Issue 3, Pages e2 (March 2010)
PRMT1 interacts with AML1-ETO to promote its transcriptional activation and progenitor cell proliferative potential by Wei-Jong Shia, Akiko J. Okumura,
Volume 141, Issue 6, Pages (December 2011)
Volume 5, Issue 6, Pages (December 2013)
Glucose-Induced β-Catenin Acetylation Enhances Wnt Signaling in Cancer
Volume 16, Issue 6, Pages (June 2015)
Kentaro Abe, Masatoshi Takeichi  Neuron 
Volume 35, Issue 6, Pages (September 2009)
Volume 49, Issue 4, Pages (February 2013)
Adamtsl2 targeting strategy and validation of inactivation.
Volume 123, Issue 5, Pages (December 2005)
Volume 16, Issue 1, Pages (June 2016)
Acetylation of histone 3 in MEF2C and GATA4 loci is enhanced in differentiating 22q11.2DS hiPSCs. Acetylation of histone 3 in MEF2C and GATA4 loci is enhanced.
Loss of HDAC-Mediated Repression and Gain of NF-κB Activation Underlie Cytokine Induction in ARID1A- and PIK3CA-Mutation-Driven Ovarian Cancer  Minchul.
TC-1 silencing sensitized A549 and SPC-A-1 cells to radiation therapy
Putative roles of HTT and ATXN3 in DNA repair and how HTT and ATXN3 polyglutamine expansions might lead to DNA damage and apoptosis. Putative roles of.
Lhx2 binds to the promoter regions of the Sox9, Tcf4 and Lgr5 genes in keratinocytes. Lhx2 binds to the promoter regions of the Sox9, Tcf4 and Lgr5 genes.
Geno- and phenotyping of selected keratinocyte cultures before and after conditional β-catenin gene ablation by Adenovirus-encoded Cre-recombinase. Geno-
Pie charts showing the percentage of genes for selected enriched biological functions and pathways at different time points after hyperthermic seizures.
Working hypothesis of histone lysine crotonylation in kidney injury.
Proposed model of mandibular dysmorphogenesis in prenatal development of Fgfr2+/S252W mice. Proposed model of mandibular dysmorphogenesis in prenatal development.
Effects of ICER deficiency on MB-cAMP-induced activation of PKA-CREB-VEGF signaling and angiogenesis. Effects of ICER deficiency on MB-cAMP-induced activation.
Treatment of HCMT-CMs with bisoprolol.
Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.
Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.
GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.
HSP90β interacts with HNF4A to regulate protein half-life.
Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.
Distribution and extent of expression.
Altered HIF-1α dynamics contribute to reduced VEGF expression in diabetic SIECs. (Aa–c) Total cell contents of HIF-1α, pAkt (at residue S473) and pS6 (at.
RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic acid. RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic.
The genomic distribution of essential and non-essential mouse genes, separated into known and predicted essentiality. The genomic distribution of essential.
TWEAK increases histone crotonylation in kidney tubular cells.
2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft. 2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft.
Oral administration of CX-4945 suppresses Tau phosphorylation in DYRK1A-overexpressing mice. Oral administration of CX-4945 suppresses Tau phosphorylation.
Fig. 4. Perinuclear dynein regulators are not required for primary ciliogenesis.RPE cells were transfected with siRNA and either nocodazole-treated, fixed,
WNT1 and WNT7B synergistically upregulate canonical Wnt target genes in YCC11 cells. WNT1 and WNT7B synergistically upregulate canonical Wnt target genes.
Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.
Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.
BRG1 interacts with RAD52 and regulates its accumulation at DSB sites during homologous recombination repair. BRG1 interacts with RAD52 and regulates its.
Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,
Peroxisome speeds were slower in patient and control cells.
Fig. 2. Acetylation stiffens primary cilia.
Nat. Rev. Rheumatol. doi: /nrrheum
Effects of paclitaxel and vinblastine.
BCL-3 regulates expression of stemness-associated Wnt targets.
Effect of siRNA transfection on colony formation by Renca cells.
Expedited loss of MacroH2A
Active Repression of Antiapoptotic Gene Expression by RelA(p65) NF-κB
JAK inhibitors suppress IL-17–induced expression of IκB-ζ.
Loss of CTCF causes p53-dependent transcriptional activation of Puma.
Epigenetic regulation of p16INK4a in human gastric cancer.
Volume 2, Issue 3, Pages (September 2012)
Competition between FLYWCH1/β-catenin and TCF4/β-catenin complexes for their interaction to Tcf-DNA-binding sites. Competition between FLYWCH1/β-catenin.
PELP1 regulates the expression and activities of MMPs in ER-negative cells. PELP1 regulates the expression and activities of MMPs in ER-negative cells.
Confirmation of candidates from WT1 binding site screen.
GPC3 promotes hepatocellular carcinoma growth by stimulating the canonical Wnt pathway (A-B) GPC3 induces the stabilization of cytoplasmic β-catenin. GPC3.
Knockdown of ERBB3, NRG1, or ERBB2 in H358-TwistER cells reduces basal PI3K–AKT signaling and ablates serum-independent proliferation before EMT. A and.
Volume 41, Issue 4, Pages (February 2011)
High nuclear β-catenin levels confer resistance to AKT inhibition and coordinates with increased nuclear FOXO3a to promote metastasis in colon cancer.
DDX5 localizes to promoters of DNA replication genes and functions in the loading of RNA polymerase II onto these promoters. DDX5 localizes to promoters.
A B C Name Sequence TIMP3 promoter
CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.
Presentation transcript:

BCL-3 does not mediate β-catenin activity through promoting nuclear translocation or altering levels of LEF1. BCL-3 does not mediate β-catenin activity through promoting nuclear translocation or altering levels of LEF1. (A) Nuclear/cytoplasmic enrichments of LS174T and SW1463 cells following BCL-3 suppression, showing localisation and expression of the Wnt transcriptional regulators β-catenin, TCF4 and LEF1. Western analysis confirms BCL-3 suppression. α-tubulin and lamin A/C serve as loading controls and confirm cytoplasmic/nuclear enrichment. WCL, whole cell lysate; C, cytoplasm-enriched lysate; N, nuclear-enriched lysate. (B) LGR5 expression following LEF1 suppression with 25 nM and 50 nM siRNA in SW1463 cells. Data shows 48 h post-siRNA transfection. α-tubulin serves as a loading control. (C) ChIP analysis of three sites in the LGR5 promoter in SW1463 cells for presence of TCF4 and BCL-3. H2O serves as a negative control for PCR. IgG serves as a negative control for immunoprecipitation (IP). Acetylated histone H3 serves as a positive control for IP. GPRC5A DS primers were designed to a downstream region in the GPRC5A gene with no reported transcription-factor binding and serves as a negative primer control. Danny N. Legge et al. Dis. Model. Mech. 2019;12:dmm037697 © 2019. Published by The Company of Biologists Ltd