Multiplexable paper-based platform for bio-analyte detection Poster # Session name Day Multiplexable paper-based platform for bio-analyte detection Sampad Laha 1, Shantimoy Kar 2 ‡ , Tapas Kumar Maiti 3, Suman Chakraborty 1* 1Department of Mechanical Engineering, Indian Institute of Technology Kharagpur, Kharagpur-721302. 2Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur-721302. 3Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur-721302. *Contacts: suman@mech.iitkgp.ernet.in Objective : Simultaneous detection and quantification of glucose and bilirubin on a paper platform Device fabrication by origami paper cutting Whatman 1 filter paper as raw material Chemical reagent immobilization Simultaneous quantification of glucose and bilirubin from plasma Visible colour changes: Brown denotes Glucose Greenish yellow denotes Bilirubin Image acquisition using Digital camera Post processing of images in Image J For Glucose : Glucose oxidase, peroxidase , iodide For Bilirubin : Diazotized sulfanilic acid, Caffeine-benzoate-acetate mixture, NaOH Testing with human plasma samples Validation with gold standard laboratory methods Results show good matching with laboratory methods (within 10% error band) Development of calibration curves using artificial standards Major Advantages Point-of-care detection Extremely low cost diagnostics Simple fabrication process Good accuracy Ability of multiplexing Glucose Calibration curve Bilirubin Calibration curve Reference : “Diagnostics for the Developing World: Microfluidic Paper-Based Analytical Devices” by Andres W. Martinez, Emanuel Carrilho, Scott T. Phillips and George M. Whitesides , Anal. Chem., 2010, 82 (1), pp 3–10 ‡ Currenly working as Postdoctorate reaserch associate at Technische Universitat Darmstadt, Darmstadt, 64287 Germany Disclaimer: The presentation of this material does not imply any endorsement or recommendation by WHO. WHO disclaims any and all liability and responsibility whatsoever for any injury, death, loss, damage, use of personal data, or other prejudice of any kind whatsoever that may arise as a result of, or in connection with the procurement, distribution and/or use of any technology presented in the 4th WHO Global Forum on Medical Devices.