CD151-knockdown cells show an aberrant spreading response to PMA

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CD151-knockdown cells show an aberrant spreading response to PMA CD151-knockdown cells show an aberrant spreading response to PMA. (A) MDA-MB-231 cells, stably expressing control vector or CD151 shRNA, were allowed to spread on plastic surfaces coated with laminin-1, for 1 hour, and then for 1 additional hour in the presence of 20 ng/ml PMA or 10 ng/ml EGF. Cells were then photographed. CD151-knockdown cells show an aberrant spreading response to PMA. (A) MDA-MB-231 cells, stably expressing control vector or CD151 shRNA, were allowed to spread on plastic surfaces coated with laminin-1, for 1 hour, and then for 1 additional hour in the presence of 20 ng/ml PMA or 10 ng/ml EGF. Cells were then photographed. Results from two independent experiments are shown for the PMA treatment. Scale bar: 20 μm. (B) The percentage of cell spreading for each condition was determined. Values are means ± s.d. n=3; *P<0.002, t-test. The difference between the EGF-treated samples is not significant. Xiuwei H. Yang et al. J Cell Sci 2012;125:1478-1487 © 2012.