CD151 effects on mammary cell morphology and adhesion.

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CD151 effects on mammary cell morphology and adhesion. CD151 effects on mammary cell morphology and adhesion. (A) MDA-MB-231 cells treated with siRNAs were seeded at 5×104 cells/well on Matrigel-coated 48-well plates and photographed after 18 hours. Scale bar: 5 mm. Results are representative of many experiments. Numbers indicate nodes with three or more branches. (B) MDA-MB-231 cells were treated with siRNAs for 5 days, and then the surface expression of α6 integrin and CD151 was determined by flow cytometry, counting at least 5000 cells/experiment. MFI, mean fluorescence intensity. (C) After treatment with siRNAs for 5 days, MDA-MB-231 cells were seeded in triplicate into 96-well plates precoated with ECM proteins. After 45 minutes, static cell adhesion was assessed. Values are means ± s.e.m.; n=3; *P<0.02. (D) After siRNA treatment the MCF-10A cells were seeded in triplicate on the indicated ECM proteins for 30 minutes, and then static cell adhesion was assessed. Xiuwei H. Yang et al. J Cell Sci 2012;125:1478-1487 © 2012.