Relative gene expression levels in MIN6 cells.

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Relative gene expression levels in MIN6 cells. Relative gene expression levels in MIN6 cells. MIN6 cells transfected with Id1 ON-TARGETplus SMARTpool siRNA or control Non-Targeting siRNA were treated with either 0.92% BSA alone or 0.92% BSA coupled to 0.4 mmol/L palmitate (Palm) for 48 h. Total RNA was extracted, reverse transcribed, and relative expression of the genes indicated determined by PCR for control siRNA-transfected cells treated with BSA (striped bars) or BSA-coupled palmitate (white bars) and Id1 siRNA-transfected cells treated with BSA (hatched bars) or BSA-coupled palmitate (black bars). Results are expressed as a percentage of mRNA levels in control (Con) siRNA-transfected cells treated with BSA (n = 4–7 in each group). *P < 0.05, **P < 0.01 for effect of palmitate treatment in control siRNA- and Id1 siRNA-transfected cells, †P < 0.05, and ††P < 0.01 for effect of Id1 siRNA in palmitate-treated cells. Mia C. Åkerfeldt, and D. Ross Laybutt Diabetes 2011;60:2506-2514 ©2011 by American Diabetes Association